溶菌酶纤维聚集体和非纤维多聚体结构的红外光谱的研究.pdfVIP

Classified Index: COAD:100075 U.D.C No A Dissertation for the Degree of M. Science Structure of Fibrillar and Non-fibrillar Aggregates of Hen Egg White Lysozyme by FTIR Spectroscopy Candidate: LI Yiyi Supervisor: MA Gang Academic Degree Applied for: Master of Science Specialty: Physical Chemistry University: Hebei University Date of Oral Examination: June, 2013 摘 要 摘 要 蛋白的纤维化过程与包括帕金森病和阿尔茨海默症等在内的许多疾病 密切相关，研究蛋白纤维化过程对这类疾病的预防和治疗具有十分重要的意 义。本文以卵清溶菌酶这一蛋白纤维化研究中常用的模型体系为研究对象， 利用红外光谱技术在酰胺 I 带对于蛋白二级结构的高度敏感性，深入研究了 溶菌酶纤维化和多聚化过程中蛋白二级结构的变化。我们应用红外光谱技术 并结合二阶导数谱和光谱分峰拟合技术跟踪了溶菌酶纤维化过程中蛋白二 级结构的变化，发现溶菌酶纤维化过程是一个蛋白原有结构逐步破坏和纤维 β-折叠结构逐步形成的过程 ，还发现溶菌酶纤维化体系在室温下能够形成非 纤维多聚体，并应用红外差谱技术研究了此多聚体的结构，发现此多聚体二 级结构为反平行的 β-折叠结构。此外，对于红外光谱定量分析蛋白二级结构 研究中水汽影响的技术问题，我们提出了一种新颖的评估思路。 关键词 溶菌酶 纤维化 多聚体 红外光谱 蛋白二级结构 Abstract Abstract Fibrillation of proteins is highly relevant to a variety of human diseases, including Parkinson’s disease and Alzheimer’s disease. Ther efore, the investigation of fibrillation process is of great significance to the prevention and treatment of these diseases. Hen egg white lysozyme (HEWL) is a widely used model protein in protein fibrillation. In this study the fibrillation and oligomerization processes of HEWL are studied using FTIR spectroscopy by taking advantage of