稻属不同染色体组着丝粒BAC克隆的分离和鉴定.pdfVIP

HEREDITAS (Beijing) 2007 7 , 29(7): 851―858 ISSN 0253-9772 研究报告 DOI: 10.1360/yc-007-0851 BAC 裔传灯, 龚志云, 梁国华, 王飞华, 汤述翥, 顾铭洪 , , 225009 : 着丝粒在真核生物有丝分裂和减数分裂染色体正常的分离和传递中起着重要的作用。通过构建 5 个稻属 二倍体野生种的基因组BAC 文库, 采用菌落杂交和FISH 技术, 筛选和鉴定了各染色体组着丝粒克隆, 并且分 析了这些克隆在不同基因组间的共杂交情况, 结果表明: (1) C 染色体组的野生种O. officinalis 和F 染色体组的 野生种O. brachyantha 具有各自着丝粒特异的卫星DNA 序列, 并且O. brachyantha 着丝粒还具有特异的逆转座 子序列; (2) A、B 和E 染色体组的野生稻O. glaberrima 、O. punctata 和O. australiensis 着丝粒区域都含有与栽 培稻着丝粒重复序列CentO 和CRR 同源的序列; (3) C 染色体组野生稻O. officinalis 的2 条体细胞染色体着丝 粒具有 CentO 的同源序列, 同时也发现其所有着丝粒区域都包含栽培稻CRR 的同源序列。这些结果对克隆稻 属不同染色体组的着丝粒序列、研究不同染色体组间着丝粒的进化关系和稻属不同着丝粒DNA 序列与功能之 间的关系均具有重要意义。 : 野生稻; 着丝粒; 细菌人工染色体; 荧光原位杂交 Isolation and characterization of the centromeric BAC clones from differnet genomes in genus Oryza YI Chuan-Deng, GONG Zhi-Yun, LIANG Guo-Hua, WANG Fei-Hua TANG Shu-Zhu, GU Ming-Hong Key Lab of Plant Functional Genomics, Ministry of Education, Yangzhou University, Jiangsu Province Key Lab of Crop Genetics and Physiology, Yangzhou 225009, China Abstract: Centromeres play an important role in ensuring the correct segregation and transmission of chromosome during mitosis and meiosis in eukaryoyes. In this research, we constructed five BAC libraries for dilpoid wild rice with different genomes. Together with the technique of colony blot hybridization and fluorescence in situ hybridization (FISH), centro- mere-related BAC clones were screened and characterized from different genomes. Meanwhile, co-hybridization was de- tected between these clones and the five genomes. The results from this study demonstrated that: (1) there were centro- mere-specific satelli