基因工程菌枯草芽孢杆菌GEB3产生的脂肽类抗生素及其生物活性研究.pdfVIP

2003, 36( 12) : 1496- 1501 Scientia Agricultura Sinica GEB3 1 1 2 2 1 高学文 , 姚仕义 , Huong Pham , Joachim Vater , 王金生 1 2 ( / , 210095; , 10587) : 利用MA DI-TOF-MS 技术, 鉴定了将pl aB3 基因转入枯草芽孢杆菌Bacillus subtilis 168 菌株所构建 工程 菌GEB3 产生 脂肽类抗生素种类结果表明, GEB3 仅产生表面活性素(surfactin) 1 种脂肽类抗生素经 C-MS 分 析, GEB3 产生由1314 和15 个碳原子 脂肪酸链构成 标准表面活性素变异体(standard surfactin isoforms) 生物活 性检测表明, 该工程菌产生 脂肽类抗生素表面活性素具有抑制小麦纹枯病菌和稻瘟病菌菌丝生长 作用 : 工程菌GEB3; 基质协助激光解吸附离子化-飞行时间质谱; 液相色谱-质谱; 脂肽类抗生素; 表面活 性素; 植物病原真菌; 抑制作用 Lipopeptide Antibiotics Produced bythe Engineered Strain Bacillus subtilis GEB3 and Detectionof Its Bioactivity 1 1 2 2 1 GAO Xue-wen , YAO Shi-yi , Huong Pham , Joachim Vater , WANG Jin-sheng 1 ( Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture/ Nanjing Agricultural niversity, Nanjing 210095; 2Institutf r Biochimie und Melikulare Biologief r Berlin Technische niversitt, BerlinD-10587, Germany) Abstract: MA DI-TOF-MS technology was used for identification of lipopeptide antibiotics produced by GEB3 strain, a derivative of Bacillus subtilis 168 which transformed by lpaB3 gene. The result showed GEB3 produced only lipopeptide antibiotic surfactin. The analysis by C-MS demonstrated that GEB3produced standard surfactin isoformswith side chain lengths of 13, 14 and 15 carbon atoms. The bioactivity detection of the surfactin indicated that the surfactin produced by GEB3 had inhibition effect to plant pathogens Pyricularia oryzae and Rhizoctonia solani.