fC31整合酶系统介导的位点特异性整合研究进展.pdfVIP

HEREDITAS (Beijing) 2011 6 , 33(6): 567―575 ISSN 0253-9772 综述 DOI: 10.3724/SP.J.1005.2011.00567 φC31 整合酶系统介导的位点特异性整合研究进展 马晴雯 , , 200040 来源于链霉菌(Streptomyces) 噬菌体φC31 的整合酶可介导链霉菌附着位点(attB)和噬菌体附着位点(attP) 之间的同源重组, 这种重组亦可在多种动植物细胞内进行; 而且, 该整合酶还可介导含 attB 位点的载体以位点 特异性方式整合于多种真核生物基因组内的假attP 位点, 并使转基因持续高效表达。因此, φC31 整合酶在基因 修饰、基因治疗及转基因动物研制等方面得到了广泛的应用。文章就近年来φC31 整合酶整合规律、提高效率 方面的改进及安全性等相关领域的研究进展进行了综述。 φC31 整合酶; 位点特异性整合; 基因治疗; 转基因; 安全性 Progress of φC31 integrase system in site-specific integration MA Qing-Wen Children’s Hospital of Shanghai, Institute of Medical Genetics, Shanghai JiaoTong University, Shanghai 200040, China Abstract: Integrase of phage φC31 catalyses the homologous recombination between Streptomyces attachment site attB and the phage attachment site attP. Meanwhile, this integrase can mediate integration of attB-containing donor plasmids into the pseudo attP sites in eukaryotic genomes by a site-specific manner and resulting long-term and robust expression of integrated genes. Nowadays, φC31 integrase system is becoming a potential tool for genome modification, gene therapy and transgenic research. Recent progress of φC31 integrase system in integration mode in mammalian genomes, efficiency im- provement and researches concerned on transgenic safety were summarized in this review. Keywords: φC31 integrase; site-specific integration; gene therapy; transgenic; safety φC31 attB attP φC31 [1]φC31 φC31 1 φC31 [2], φC31 Cre FLP 1.1 , φC31 φC31 attB 收稿日期: 2011−01−17; 修回日期: 2011−04−06 基金项目: (2009ZX08010-018B) 作者简介: , , , Tel: 021 E-mail: maqingwen@ 致 谢: , 网络出版时间: 2011-4-19 9:20:54 URL: /kcms/detail/