蛋白质水力学半径的测定及用于蛋白质变性过程的实时监控.pdfVIP

59 6 Vol1 59 No1 6 2008 6 Journal of Chemical Industry and Engineering ( China) June 2008 2 白丽燕, 陈春琴, 林东强, 姚善泾 ( , 3 0027; 2 , 3 0002) : Zetasizer Nano ( BSA) , pH , BSA pH , BSA / U0 ; , , , BSA ; pH , , , BSA , , SDS BSA T BSA , ; DTT m , BSA , , : ; ; ; ; : T Q 93 : A : 0438- 57 (2008) 06- 485- 05 Measurement of protein hydrodynamic radius and it s applicat ions to monitor protein denaturation 2 BAI Liyan , CHEN Chunqin , LIN Dongqiang , YAO Shanjing ( Dep artment of Chemical and B iochemical E ngineering, Zhej iang University , H angz hou 3 0027, 2 Zhej iang, China; H uadong Medicine Co1 , td1 , H angz hou 3 0002, Zhej iang, China) Abstract: T he hydrodynamic radius ( Rh ) of bovine serum albumin ( BSA ) w as measured w ith the Zetasizer Nano instrument1T he influences of pH, ionic strength and surfactants w ere investigated, and the real-time measurement of BSA size w as used to monitor the thermal denaturation and urea denaturation of BSA1 The results indicated that Rh show ed a typical / U0 form w ith the increase of pH1 In acid solution, w ith increasing ionic strength the Rh decreased a little first, and then increased remarkably1At neutral pH the in