《HPLC和GC-MS检测两种提取法所得当归挥发油的化学成分》.pdfVIP

谢静1，一，张浩“，苏丹2，易涛2 (1．成都医学院药学系，四川成都610083；2．四川大学华西药学院，四川成都610041) 摘要：目的 比较两种提取方法所得挥发油的化学成分。方法 当归药材用水蒸气蒸馏和石油醚提取，用HPLE和Gc—MS 石油醚法所得苯酞类化合物的种类比水蒸气蒸馏法的多。后者提取的为小分子易挥发的烯萜类成分，与文献报道相同。石 油醚法收率是水蒸气蒸馏提取的2．5倍。结论两种提取法所得挥发油化学成分有差异，石油醚提取当归挥发油较好。 关键词：当归；挥发油；化学成分；高效液相色谱法；气相色谱一质谱联用法 中图分类号：R917 文献标识码：A Detectionofessentialoilof sinensisobtainedwithdifferentextractionmethods Angelica by ofHPLCandGC．-MS combination XIE Ta02 Jin91”，ZHANGHao“，SUDan2，YI Medical ChinaSchool Univer- (1．PharmacyDepartmentofChengduCollege，Cher曙du610083，China；2．WestofPharmacy，Sichuan sity，Chengdu610041，China) To and thechemical ofessentialoilextractedfromRadix sinensis Abstract：OBJECTIVEanalyzecompare composition Angelicae 诵thdifferentmethods．METHODSHPLCandGC—MSwereused．RESUI。TS 0fessentialoilextracted 100％components by steamdistillationwereidenticalwith ofthat benzinedetermined ofessential 50％componentsbypetroleum byHPLC．34％components oilextractedsteamdistillationwereidentiealto ofthatthe benzinedeterminedGc—MS．-11leex． 43％components by by petroleum by tractfrom benzinecontainedmore thanthatfromsteamdistillation．andthe ofconstituentsintlleexo petroleum phthalides majortypes tractfromsteamdistillationwere asafewesters．CONCLUSIONThechemical varieswithdif- alkenes．terpenes舶well composition ferentextractionmethods．Theresults themethodwiththe benzineextractioniSmore suggest petroleum satisfactory． sinensis；Essentialoil；Chemical Keywords：Angelica composition；HPLc；GC—MS CLCnumber：R917 Documentcode：A A