QseC controls biofilm formation of non-typeable Haemophilus influenzae in addition to an AI-2-dependent mechanism》.pdf

International Journal of Medical Microbiology 302 (2012) 261–269 Contents lists available at SciVerse ScienceDirect International Journal of Medical Microbiology j o u r n a l h o m e pa ge: w w w. elsev ier.de/ijmm QseC controls biofilm formation of non-typeable Haemophilus influenzae in addition to an AI-2-dependent mechanism Can M. Ünal a , Birendra Singh a , Christophe Fleury a , Kalpana Singh a , Luis Chávez de Paz b , Gunnel Svensäter b , Kristian Riesbeck a,∗ a Medical Microbiology, Department of Laboratory Medicine Malmö, Lund University, Skåne University Hospital, Malmö, Sweden b Department of Oral Biology, Malmö University, Malmö, Sweden a r t i c l e i n f o a b s t r a c t Article history: Non-typeable Haemophilus influenzae (NTHi) is a common pathogen associated with diseases such as Received 8 November 2011 acute otitis media or exacerbations in patients with chronic obstructive pulmonary disease. The biofilm- Received in revised form 2 June 2012 forming capability substantially contributes to the persistence of NTHi. However, the regulation of biofilm Accepted 8 July 2012 formation is not completely understood. Quorum sensing regulated by autoinducer-2 produced by luxS is until now the only described regulatory mechanism. In this study, we show that the two-component Keywords: signalling system QseB/C is involved in the biofilm formation of NTHi in vitro. An isogenic NTHi mutant Biofilm of qseC (Hi3655KR2) showed a significant decrease