Quantitative significance of functional genes of methanotrophs and propanotrophs in soil above oil and gas fields, China》.pdf

Journal of Petroleum Science and Engineering 120 (2014) 170 –176 Contents lists available at ScienceDirect Journal of Petroleum Science and Engineering journal homepage: /locate/petrol Quantitative significance of functional genes of methanotrophs and propanotrophs in soil above oil and gas fields, China Cui-Yun Zhang n, Ze He, Sheng Zhang, Mi-Ying Yin, Zhuo Ning, Ya-Ci Liu Institute of Hydrogeology Environmental Geology, Chinese Academy of Geology Sciences, Shijiazhuang 050061, China a r t i c l e i n f o a b s t r a c t Article history: To assess whether the anomalies of methanotrophic pmoA and propanotrophic prmA genes as target Received 19 January 2014 genes can reflect the existence of underlying oil and gas reservoirs, natural abundance and distribution Accepted 15 June 2014 of target genes were investigated in soil above known gas fields, oil fields and non-oil and gas fields, Available online 24 June 2014 China. A total of 45 soil samples were collected from different depths of 2.5-m soil profiles in Xiaoquan Keywords: gas field, Xiliu oil field and Beiguan non-oil and gas field and from 50 cm depth of the old oil area, the methanotrophic pmoA gene new developed area, the oil producing area and the undeveloped area of Xiliu oil field, respectively for propanotrophic prmA gene analysis of target genes by SRBR Green-based fluorescent quantitative real-time polymerase chain quantitative RT-PCR