Quantum dot-modified aptamer probe for chemiluminescence detection of carcino-embryonic antigen using capillary electrophoresis》.pdf

Sensors and Actuators B 210 (2015) 158–164 Contents lists available at ScienceDirect Sensors and Actuators B: Chemical j o u r n a l h o m ep a g e: w w w. elsev /locate/snb Quantum dot-modified aptamer probe for chemiluminescence detection of carcino-embryonic antigen using capillary electrophoresis Zi-Ming Zhou a,1, Zhe Feng a,1, Jun Zhou b , Bi-Yun Fang a , Zhi-Ya Ma a , Bo Liu a , Yuan-Di Zhao a,∗ , Xue-Bin Hu c,∗ a Britton Chance Centerfor Biomedical Photonics at Wuhan National Laboratory for Optoelectronics – Hubei Bioinformatics Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China b Department of Clinical Laboratory, Wuhan Commercial Staff Hospital, Wuhan 430021, PR China c Union Hospital, Tongji Medical College of Huazhong University of Science Technology, Wuhan 430022, PR China a r t i c l e i n f o a b s t r a c t Article history: A new method is proposed for determination of carcino-embryonic antigen (CEA) based on quantum Received 19 June 2014 dot (QD)-modified aptamer probe by capillary electrophoresis-chemiluminescence (CE-CL) detection Received in revised form system. An oligonucleotide sequence A contains two parts, one is CEA aptamer and the other is bridging 17 December 2014 segment. Horseradish peroxidase (HRP) conjugates with sequence A (HRP-DNAA ) and then mixes with QD Accepted 20 December 2014 Available online 29 December 2014 labeling complementary bridging segment (QD-DNAB ), forming HRP-DNAA-