Radiotracer studies on molecular mechanisms of death and resuscitation》.pdf

lnrernational JournalofApplied Radiation andIsoropes.Vol.31. pp. 707 lo 711 0 Pergamon Press Ltd 1980.PrintedinGreatBritain Radiotracer Studies on Molecular Mechanisms of Death and Resuscitation VASILII J. NIKULIN, ANTONINA V. POGOSSOVA and ANNA S. KONIKOVA Central Institute Advanced Training for Physicians, 2, Barrikadnaya St., Moscow, U.S.S.R. (Received 20 August 1979 in revised form 19 September 1979) Tracer techniques and artificial circulation were applied to rabbits after death by anoxia and deep hypothermia in order to study molecular mechanisms. 60 min after death the biosynthesis and disinte- gration of protein, RNA and DNA practically stopped in all organs. In animals cooled post mortem the process of biosynthesis and degradation of protein, RNA and DNA, as well as the physiological func- tions of the whole organism, were restored. Introduction ibility of post mortem alterations of metabolism, the starting mechanisms of recovery metabolism and viial THE ISOTOPIC tracer method enriched biology with activity of the whole organism. Connected with the many new facts and concepts. It proved especially knowledge that at deep hypothermia the cessation of fruitful in studying the processes occurring at the mol- vital activity is regularly reversible, we studied metab- ecular level