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QTL Mapping of Vernalization Requirement in Brassica rapa.pdf
AgriculturalBiotechnology 2015,4(4):1—5 PlantScience
QTLMappingofVernalizationRequirementinBrassica
rapa
YatingLIU,LeiZHANG,XiaohongJI,XixiLV,YugangW ANG ,HuiFENG
CollegeofHorticulture,LiaoningProvincialKeyLaboratoryofGeneticsandBreedingofCrueifcreusVegetableCrops,ShenyangAgriculturalUniversity,Sheny—
ang110866,China
Abstract AnF,population。derivedfrom aCroSSbetweentheverBalizationindependcntgenotype ‘RcBr’andthevenlalizationdependentgenotype ‘08A061’,
wasdevelopedtoconstructalinkagemapwith165SSRandInDelmarkers.QTLanalysiswasperformedbytwophenotypicevaluation(daysto5emelongatestalk
nadfloweringtime)basedonthedifiefenceinF,1familiesunderVeITIalizationnadnoverBalization.TheyesultsshowedthattheveBralizationrequirementwasre-
cessiveinBrassicarapa.SevenQTLsthatcontrolledveotalizationreuqirementweredetectedonA02andA06linkagegroups,whichexplainde phenotypicvariation
Ⅲ ngfrom0.08% to22.52%.TwoQⅡ (VR—DEOl,VR.FI1D1)weledetectedonthetopofA02,whichexplainde 22.52% and14.54% 0fthephcnotypic
variation,respectively.nadcloselylinkedwithBrFLC2gene.TwoQTl暑(VR—DE03。VR—DEo4)weredetectedonAo6with13.30% nad13.64% ofPhenotypic
variation.Thesedetected0rrL6wil1provideusefulinformationonunderstandingthegeneticbasisbetweenVelTIalizationrequirementandbolting。nadthatwi11be
usefulformarker-assistedselection(MAS)inabreedingprogram forboltingresistantcultivars.
Keywords Brassicarapa:Vernalizationreuqirement:Qunatitativetraitlocus(QTL)
Brassica cropscanbeclassified intoannualsandbiennials DH populationofB.rapaanddemonstratedthattheO11Lwas
accordingtotherequirementforaperiodoflow—temperature in— closelylinkedwithB C2gene.Onthecontrary.thestudyon
ductionbytheirflowering -2j.A biennialvarietyrequiresaperi— BrassicaoleraceanadBrassicanapusshowedthatvernalizationre.
odsoflow—temperature vernalization to enablethe induction of quirement
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