《Putrescine Plays a Positive Role in Salt-Tolerance Mechanisms by Reducing Oxidative Damage in Roots of Vegetable Soybean.》.pdf

AvaiJabIeonlineatwvc~v．sciencedirect．corn PutrescinePlaysa PositiveRole inSalt-ToleranceMechanismsby ReducingOxidativeDamageinRootsofVegetableSoybean ZHANGGu。wen，XUSheng—chun，HUQi。zan，MAOWei—hua2andGONGYa—ming InstituteofVegetable，ZhejiangAcademyofAgriculturalSciencesHangzhou310021，P．R．China CenterofAnalysisandMeasurement,ZhejiangUniversity,Hangzhou310029,P．R．China Abstract Polyaminesplay importantrolesinplanttolerancetoenvironmentalstress．W iththeaim ofinvestigatingthepossible involvementofputrescine(Put)insalt—tolerancemechanismsinvegetablesoybeanroots，exogenousPut(10mmolL)‘‘ anditsbiosyntheticinhibitorD-arginine(D—Arg)(0．5mmolL)wereaddedtonutrientsolutionwhenvegetablesoybean (GlycinemaxL．cv．Huning95-1)seedlingswereexposedto100mmolL sodiumchloride(NaC1)．Theresultsshowed thatPutamelioratedbutD-ArgaggravatedthedetrimentaleffectsofNaC1onplantgrowthandbiomassproduction．Under NaC1stress，levelsoffree，solubleconjugatedandinsolubleboundtypesofPutinrootsofvegetablesoybearlwerereduced， whereasthoseofrfee，solubleconjugated，andinsolubleboundtypesofspermidine(Spd)andspermine(Spm)wereincreased． ExogenousPuteliminatedthedecreaseinPutbutpromotedtheincreaseofSpdandSpm．However，thesechangescouldbe reversedbyD—Arg．UnderNaC1srtess，activitiesofargininedecarboxylase(ADC)，S-adenosylmethioninedecarboxylase (SAMDC)，diamineoxidase(DAO)，andpolyamineoxidase(PAO)wereinduced，withexogenousPutpromotingandD-Arg reversingthesechanges．Furthermore，NaC1stressdecreasedactivitiesofantioxidantenzymes．ExogenousPutalleviated butD-ArgexaggeratedtheseeffectsofNaC1stress，resultinginthesamechangesinmembraned~ ageandreactiveoxygen species(ROS)production．TheseresultsindicatedthatPutplaysapositiveroleinvegetablesoybeanrootsbyactivating antioxidantenzymesandtherebyattenuatingoxidativedamage． Keywords：NaCIstress，oxidativedamage，purtescine，salt—tolerance，vegetablesoybean OHSdefenseme