《Quantitative analysis of site-specific N-glycans on sera haptoglobin βchain in liver diseases.》.pdf

ActaBiochimBiophysSin2013，45：1021—1029I@TheAuthor2013PublishedbyABBSEditorialOfficeinassociationwithOxfordUniversityPressonbehalfoftheInstitute ofBiochemistryandCellBiology,ShanghaiInsttiutesforBiologicalSciences．ChineseAcademyofSciences．DOl：10．1093a／bbs／gmt110． A - Advance AccessPublication8October2013 ／ OriginalArticle Quantitativeanalysisofsite—specificN-glycansonserahaptoglobinI3；chain inliverdiseases ShuZhang，KaiJiang，ChunSun，HaojieLu，，andYinkunLiu， LiverCancerInstitute，ZhongshanHospital，KeyLabolatoryofCarcinogenesisandCancerInvasion，FudanUniversity，MinistryofEducation， Shanghai200032，China DepartmentofChemistry，FudanUniversiyt，Shanghai200433，China InstiuttesofBiomediea1Sciences．FudanUniversity．Shanghai200032．China *Correspondenceaddress．Tel：+86—21Fax：+86—21E—mail：luhaojie@fudna．edu．cn(H-J．L．)， liu．yinkun@zs．hospita1．sh．cnfL．Y．K．) Thesite--specificcharacterizationofN-glycansinglycopro-- onlyatanadvancedstagewhenlimitedtherapeuticoptions teinswiththepotentialofclinicalapplicationisimportant． areavailable 1『1．．Fetoprotein(AFP)isanestablishedbio— In our previous report，the overallN-glycans ofsera markerfortheprogression ofliverdiseasetolivercancer haptoglobin(Hp)Bchainwerefoundtobedifferentin 2『1butitisnotefficienttodistinguishvariousofrmsof liverdiseases．HpBchaincontainsfourpotentialsitesof chronicliverdiseases．Furthermore，serum AFP levelscan N-glycosylation．Inthisstudy，weinvestigatedthepotential beelevatedundermanyphysiologicalcircumstances，thusit changeofN-glycansonHp Bchaininasite-specific afofrdsrestrictedspecificitydiscriminatingthepathological fashion．SeraHpBchaininhealthyindividualsaswellas statesofliverdiseases．Theestablishmentofauxiliarydiag- patien