褪黑素光谱特性试系统设计及相关实验研究.pdfVIP

摘 要 褪黑素(Melatonin，MT)主要是由松果体合成和分泌的一种吲哚类激素，具有明显 的昼夜节律特点和调节内分泌系统、生殖系统和免疫系统的功能。最新研究发现褪黑 素能有效的抑制和治疗癫痫病。MT 通过增强癫痫状态大鼠海马谷氨酸脱羧酶活性及γ- 氨基丁酸合成发挥抗癫痫作用。 本文介绍了光电直读光谱仪的设计原理与设计方法。包括光学部分, 电路部分和PC 机软件部分:光学系统设计中，主要进行了光栅单色仪和光电倍增管的系统研究和分析, 并采用光电倍增管实现光信号转换为电信号。电路部分完成微弱信号进行的放大,滤波 和整流,，通过AD 转换电路，将模拟信号转换为数字信号后将数字信号送给单片机,单 片机完成对 AD 转换后信号的采集的同时实现对步进电机的控制并且与计算机进行串 口通信。PC 机软件部分采用Visual C++编写的Windows 控制界面,通过计算机串口接 受单片机传输过来的数据,并画出样品的吸收光谱曲线,同时完成对单片机系统的控制。 配置了5 组癫痫状态wistar 大鼠大脑皮质上清液样品,分别采用吸收光谱,拉曼光谱 和荧光光谱的分析方法，对不同组模型样品进行检测分析。检测结果表明给癫痫态大 鼠腹腔注射褪黑素后能改变其大脑皮质蛋白质性质，这种改变性质后的蛋白质具有治 疗和抑制癫痫发病的功能。 本课题得到华中科技大学科学研究基金资助。 关键词： 癫痫病 光栅光谱仪 光谱 光电倍增管 I Abstract Melatonin, a kind of hormone, plays an important and special role in physiological activities, controlling a series of physiological activities, such as procreation, incretion, immunity, neural system, et al. Melatonin has a positive effect on many diseases, such as cancer, AIDS and many brain diseases; it also can help prolong life and improve sleep. Epilepsy is one of the diseases that very difficult to treatment. Nowadays, we also do not find a good method to cure this serious disease. Luckily, the latest researches show that the Melatonin has the special function to cure epilepsy. It is a good news to epilepsy treatment. This essay clarified a testing way which is different from the traditional way of analyzing Epilepsy, presented the basic research methods of Epilepsy are medicinal methods. The results of these methods are frequently influenced by different factors which can not forecast. But spectral analysis can easily, quickly and accurately get the result. Meanwhile, the samples can be l