《Establishment of a Multi-color Genomic in situ Hybridization Technique to Simultaneously Discriminate the Three Interspecific Hybrid Genomes in Gossypium》.pdfVIP
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《Establishment of a Multi-color Genomic in situ Hybridization Technique to Simultaneously Discriminate the Three Interspecific Hybrid Genomes in Gossypium》.pdf
Journalof
Integrative尸怕爪Biology2008,50(3):345-351
EstablishmentofaMulti..colorGenomic/nsitu
to
HybridizationTechniqueSimultaneously
in
theThree Genomes
InterspecificHybrid
Guan,Kai GuoandTian-Zhen
Bing Wang,Bao-LiangZhou’,Wang-Zhen Zhang
fNational of Enhancement,CottonResearch
KeyLaboratoryCropGeneticsGermp『日sm Institute,
Nanjing University。Nanjing210095,China)
Agricultural
Abstract
and in
To alienchromosomesin to
identify recipientprogeniesanalyzegenomecomponentspolyploidy,agenomic/n
situ thatissuitableforcononwas increased
developed
hybridization(GISH)technique using stringency
increased wereacombinationofthefoMrfactorsinthe state:100:1ratioof
conditions
stnngency foIlowingoptimized
to oC anda min these
DNA washsolution.43 wash 13 wash.Under
blockingprobe。60%formamide temperature specific
conditions from a were observedon
using Gossypiumsturtianum(ClCl)asprobe.stronghybridizationsignalsonly
gDNA
chromosomesfromthe insomaticcellsofthe x
Cl
genome
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