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《High universality of matK primers for barcoding gymnosperms》.pdf
Journal andEvolution
ofSystematics 49(3)：169-175(2011)
ResearchArticle
ofmatK for
universalityprimersbarcoding
High
1,2yanLI GA04l，一,4RamC．POUDELl，3De．ZhuLi 5AlanFORREST
ILian．Ming
650204，China)
2(Institute 674100，China)
3(GermplasmBankoffieldSpeciesinSouthwestChina，KunmingInstitute
Graduate
4(The UniversityofChineseAcademyofSciences，Beijing100049，China)
Garden EH3
BotanicEdinburgh．Edinburgh5LIL
5(Royal UK)
AbstractDNA isatoolto andaccuratetaxonomicidentificationastandardDNA
barcoding providerapid using
was forland
two-markercombinationofmotK+rbcL asthecorebarcodc
region．A formallyproposed plantsby
theConsortiumfortheBarcodeofLifePlant are no
WorkingGroup．HoweveL廿1erecurrentlybarcodingprimers
formatK in used57 40
showinghighuniversalitygymnosperms．W-egymnospermspeciesrepresentinggenera．1l
the ofninecandidatematK andonerbcL inthis
familiesandfoursubclassestoevaluate
universality primers primer
asauniversalfor dueto
l here
study．Primerf F／724R)of而吐isproposed primergymnospermshighuniversality．
the for
OneoftheninecandidatematK a
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