《High-throughput Procedure for Single Pollen Grain Collection and Polymerase Chain Reaction in Plants》.pdfVIP
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《High-throughput Procedure for Single Pollen Grain Collection and Polymerase Chain Reaction in Plants》.pdf
Journal F,『a仃f
ofIntegrativeBiology2008,50(3):375--383
Procedurefor PollenGrain
Single
High--throughput
inPlants
and ChainReaction
Collection
Polymerase
Panlt。andRu-KaiChen2
Ping.HuaChenl,2t,Yong-Bao
Research Research 70360,USA;
(1USDA-ARS,SouthernRegional Center,SugarcaneLaboratory.Houma,LA
and Normal 350002.China)
FujianAgricultureForestryUniversity,FujianUniversity,Fuzhou
Abstract
jn Iira/tednumbersof
chain succeededseveraI
pollen teaction(PCR)has species,howeveronly
Single grainpolymerase
involvedduetodi怖curiesin isolationand hasIira/tedlts In
were applicationgeneticanalysis
pollengrains pollen lysis.This
for and variationina
and studiesin detecting large
mapping plants.Ahigh.throughput(Hnprocedurecollecting genetic
HT thecollectionofIndividuaI
numberofindividual PCRis involved
pollengrainsby reported.Theprocedure potiengrains
of andthe of inaheated solutionfoBowedneutralizationwith
a forceps Iysispollengrains
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