《Screening of eye-position related genes with DD-RT-PCR and RDA in the hybrids between Japanese flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus》.pdfVIP

ChineseJournalof and OceanologyLimnology V01．27 No．1，P92．99．2009 DoI：10．1007Is∞343．009-0092．1 of related with Screeningeye——positiongenesDD·-RT．．PCR andRDAinthe between flounder hybrids Japanese olivaceusandstoneflounderKareius Paralichthys bicoloratus* CHEN Yanjie(陈妍婕)，ZHANGQuanqi(张全启)”，QIJie(齐洁)，SUNYeying(孙业盈)， ZHONG Qiwang(钟其旺)，WANGXubo(王旭波)，WANGZhigang(王志刚)， LI Shuo(李朔)，LIChunmei(李春梅) Geneticsand LaboratoryofMafine Breeding,OceanUniversity 266003，China ofChina,Qingdao Received Nov．30，2007；revisionMar．3，2008 accepted AbstractFIatfishorfloundermovesone to intovertebral eye changebodyproportion asymmetry which somebecomesinistralwhileothors duringmetamorphosis,during dextral．However,the mechanismbehindsthe been hasnot wellunclerstood．Intllis between eye．position research．hybrids mixed stoneflounder(8)show inbotIldextralandsinistral Japaneseflounder(Q)and eye．10cation type thusbecome for the from type，and goodsamplesstudyingeye-migration．mRNAs pro—metamorphosis sinistraianddextral lalvaewere screenedwithclassicaldifferential hybrids displayRT-PCR(DD．RT^PCR) and difference of and47 were representationalanalysiscDNA(cDNA-RDA)；30putativefragments cDNA ofcreatinekinaseand 2 isolated isolated，respectively．Thefragments trypsinogenprecursorgenes cDNA—RDAexhibited