《Validation and Characterization of Ghd7.1, a Major Quantitative Trait Locus with Pleiotropic Effects on Spikelets per Panicle, Plant Height, and Heading Date in Rice (Oryza sativa L.)》.pdfVIP

Journal of Integrative Plant Biology 2013, 55 (10): 917–927 Research Article Validation and Characterization of Ghd7.1, a Major Quantitative Trait Locus with Pleiotropic Effects on Spikelets per Panicle, Plant Height, and Heading Date in Rice (Oryza sativa L.) †§ § * Touming Liu , Haiyang Liu , Huang Zhang and Yongzhong Xing National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China yPresent address: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China §These authors contributed equally to this work. Corresponding author Tel: þ86 27 8728 1715; Fax: þ86 27 8728 7092; E‐mail: yzxing@ Available online on 20 May 2013 at and /journal/jipb doi: 10.1111/jipb.12070 Abstract A quantitative trait locus (QTL) that affects heading date (HD) and the number of spikelets per panicle (SPP) was previously identified in a small region on chromosome 7 in rice ( Oryza sativa L.). In order to further characterize the QTL region, near isogenic lines (NILs) were quickly obtained by self‐crossing recombinant inbred line 189, which is heterozygous in the vicinity of the target region. The pleiotropic effects of QTL Ghd7.1 on plant height (PH), SPP, and HD, were validated using an NIL‐F2 population. Ghd7.1 explained 50.2%, 45.3%, and 76.9% of phenotypic variation in PH, SPP, and HD, respectively. Ghd7.1 was precisely mapped to a 357‐kb region on the basis of analysis of the progeny of the NIL‐F2 population. Day‐length treatment confirmed that Ghd7.1 is sensitive to photoperiod, with long days delaying heading up to 12.5 d. Identification of panicle initiation and development for the pair of NILs showed that Ghd7.1 elongated the ph