《中国东北条子河中微生物群落变化与锌砷污染的关系研究 Diversity change of microbial communities responding to zinc and arsenic pollution in a river of northeastern China》.pdfVIP

Jin et al. / J Zhejiang Univ-Sci B (Biomed Biotechnol) 2014 15(7):661-669 661 Journal of Zhejiang University-SCIENCE B (Biomedicine Biotechnology) ISSN 1673-1581 (Print); ISSN 1862-1783 (Online) /jzus; E-mail: jzus@ Effects of organic solvents on two retinal pigment epithelial * lipofuscin fluorophores, A2E and all-trans-retinal dimer §1 §2 3 2 †‡1,2 Qiu-xia JIN , Xin-ran DONG , Jing-meng CHEN , Ke YAO , Ya-lin WU 1 ( College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China) 2 ( Eye Center, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China) (3School of Medicine, Zhejiang University City College, Hangzhou 310015, China) †E-mail: yalinw@ Received July 19, 2013; Revision accepted Nov. 7, 2013; Crosschecked May 7, 2014 Abstract: Gene and drug therapies are being developed to alleviate vision loss in patients with Stargardt’s disease and age-related macular degeneration (AMD). To evaluate the therapeutic effects of these treatments, organic sol- vents are routinely used to extract and quantify bisretinoid lipofuscin constituents, such as N-retinylidene-N-retinyl- ethanolamine (A2E) and all-trans-retinal dimer (ATR-dimer). By high-performance liquid chromatography (HPLC), we found that A2E and ATR-dimer were both altered by tetrahydrofuran (THF) and chloroform, but were stable in d