《免疫鸭群中一株变异型番鸭呼肠孤病毒的分离与特性研究 Isolation and characterization of a variant avian reovirus from vaccinated Muscovy duck flocks》.pdfVIP

第35卷第8期 中国预防兽医学报 V01．35．No．8 2013年8月 ChineseJournalofPreventive Medicine Aug．2013 Veterinary doi：10．3969／j．issn．1008-0589．2013．08．04 免疫鸭群中一株变异型番鸭呼肠孤病毒的分离与特性研究 陈 峰，，雷 雯z，张祥斌t，招丽婵2，操胜2，刘 闯2，覃健萍2，李海燕2’ 1．华南农业大学动物科学院，广东广州510642； 2．广东温氏食品集团股份有限公司广东温氏集团研究院 技术中心 ，广东云浮527439 摘 采用MDPV阳性血清中和后，经2轮番鸭胚有限稀释克隆获得纯化的DRV。电镜观察病毒粒子呈球形、无囊膜、 双层衣壳、直径约70 5 nln。F6代病毒滴度为logl07TCID√0．1mL，病毒能够致番鸭胚成纤维细胞产生细胞病变， 并能够致死番鸭胚和SPF鸡胚，回归1日龄雏番鸭出现典型的肝脏点状或斑块状出血等特征性病变，与临床发 胚、SPF鸡胚及雏番鸭致病力强，而且其基因序列比早期分离株变异较大，应加强监测并及时筛选新疫苗株以有 效防控该病的发生和蔓延。 关键词：番鸭；呼肠孤病毒；分离；电镜；特性 中图分类号：$852．65 文献标识码：A IsolationandCharaCter．ZatiOnofavariantavian reovirus frOm vaccinatedduckflocks Muscovy CHEN Wen2，ZHANG Li—chan2，CAO Fen91，LEI Xiang—binl，ZHAO Shen92， LIU Chuan92，QIN Jian—pinga，LIHai—yan2+ China 1．SouthAgriculturalUniversity，Guangzhou510642，China； WENSFoodstuff 2．GuangDong GroupCo．Ltd，Yunfu527439，China Abstract：Aduck isolatedfromvaccinated administratedwith duck reovirus DRV was Muscovyducks，which Muscovy reovirus and duck vaccine in limiteddilutionsin Muscovyparvovirus MDPV vaccinesGuangdongprovince，andpurifiedby duck fromtheaffectedducktissue with MDPV． embryonated Muscovy eggs samplepre—treatedpolyclonalantibodyagainst Electron examinationshowedthattheisolateexhibited charactersofDRV．Theisolate the microscopy typicalmorphological cause deathof9一to1 oldof duck andSPFchicken in96hours infection．Thevirestiterat6th 1-day Muscovyembryos embryos post was 5 mL．Infectionof duckswiththeisolate clinicaland TCID5D／0．1 passagelogl07 day-oldMuscovy reproducedtypical signs lesionsseeninthe sick ofthe that was pathological originalducks．SequenceanalysisisolateshowedtheS3gene 99．9％homology withthat fromTaihuareain with ofDRV—THllisolated 2011．whereasit shared66．7％to those only 67．2％identity ofMuscovy DRVisolatedearlier．TheisolateclusteredatthesamecladewiththoseDRVisolatedinrecent theclassical years，while Muscovy DRVclusteredina distant on results the of thevirus lineagephylogeneticanalysis．Thesehighlightnecessitymonitoring o