《农杆菌介导的转化芦荟的研究 Genetic Transformation of Aloe barbadensis Miller by Agrobacterium tumefaciens》.pdfVIP

ofGeneticsandGenomics Journal at Availableonline ActaGene“ca Formerly Sinica December 2007，34 12 ：1053—1060 GeneticTransformationofAloebarbadensisMiller by Agro- bacterium tumefaciens He Chen Du Congfen 1,2，Jie 2，Yinmao1， 1～，JiaxingZhang 1,2，XingguoYe㈨，Lipu Dong 1 HuaZhao 1CollegeofChemistry Business University，Belting100037，Ch／na； 2 alent,BeOing11 0081．China aloein the of cosmeticand ofaloenloebarbadensis Abstract：Despiteimportance phanamceuticalindustries．improvement Miller wasseldom this andtransformation reported bygeneticengineering previously．Instudy,regeneration conditions，includingexplant selectionandsurface ofdifferent co-cultore Theuseof sterilization．use strains，and Agrobacterium processing，areoptimized 20．O％sodium sterilizationwasIessdetrimentalto thall thehealth 01％mercuricchloriden0 hypochlofide 25min for ofexplant fromstemswagmuch than min ．Regenerationfrequency higherthatfromleavesor wereinfected sheaths．ExplantsbyAgmbacte— rium 30 co-culturalmedium，andthiswasfoIlowedthree co-cultureonsterilefrlter with for10h min inliquid by days paperslight at24。C．Histoehemiealdatademonstratedthatthetransient ofGUS intllestem ofa10einfected perday expressiongene explants with strainsEHAl05andC58C1wes80．0％and the oftheex— Agrobacterium 30，0％。respectively,suggestinghigllersensitivity toEHAl05thantoC58C1．Infectedtissueswereselected ihansforraants． plants o usingG418 10，O_25me／L togenerate G418resistant fromthe were infected ELISA indi— Sixty—sevan planfletsgenerated explants．Southern analyses blotting。PCR，and caredthatthealien aloe were transferredinto andwas inthe 11lis established geae successfully expressed Wansgenicplants newly transformation system couldheusedforthegeneticimprovementofaloe． analysis AloerAlee barbadensisconsideredas Genetic canbeusedforthe MiHer is engineering improvement a candidatefor in the researchthenewCeil． ofaloe．ToOUr prime plant concerning knowledge，reports ofits medicinalandeco- mry,becausesignificant tr