《利用响应面法优化北冬虫夏草发酵米固态发酵条件 Optimization of Solid-state Fermentation of Rice by Cordyceps militaris by Response Surface Methodology》.pdfVIP

265 ※生物工程 食品科学 2013,V01．34,No．19 利用响应面法优化北冬虫夏草发酵米 固态发酵条件 王永显1，刘岩1，姜鹏1，江健健1，朱一明1，马丽琨2 (1．青岛市食用菌工作站，山东青岛 266071：2．青岛种子服务中心，山东青岛266071) 摘要：采用Plackett．Burman设计筛选出对北冬虫夏草发酵米有显著影响的3个因素：料水比、光照强度、光照时 间；在此基础上进行最陡爬坡试验确定试验的最大响应区域，根据中心组合的原理设计响应面试验，得到北虫草 9．19d，虫草素含量为64．1mg／lOOg。 关键词：响应面法；北冬虫夏革发酵米：虫革素 ofSolid—stateFermentationofRice militaris Surface Optimization byCordycepsbyResponseMethodology WANG Yahl，JIANG Yong-xianl，LIU Yi-min91，MALi-kun2 Pen91，JIANGJian-jianl，ZHU Edible SeedService (1．QingdaoFungusWorkstation，Qingdao266071，China；2．QingdaoCenter,Qingdao266071，Chin) Abstract：Plackett—Burmanwas toscreen3 and designapplied significantones(material／waterratio，lightintensity 5 factors fermentationofrice militaris．Afar illumination thesolid-state time)frompotentialkey influencing byCordyceps themaximum was ascent factorswere responseregionapproachedbysteepestpathdesign，thesignificant o硼mizedby central combinedwith surface material／waterratioof compositedesign responsemethodology．A 5．19：1(m，功，temperature sizeof12mL／100 of and of d tothe of25℃，inoculum 980．13Ix anilluminationtime9．19werefound g，lightintensity conditionstoobtaina of 64．1 optimum cordycepinyield medl00g． surface militarisfermented Keywords：responsemethodology；Cordycepsflee