胃癌细胞原代培养及Ming氏浸润型胃癌细胞系建立.pdf

万方数据 目 录 缩写词表································································································1 中文摘要································································································2 英文摘要································································································3 引 言································································································5 材料和方法·····························································································6 一、材料································································································6 1．胃癌标本······················································································6 2 ．实验动物······················································································6 3 ．主要仪器设备················································································6 4 ．主要试剂······················································································7 5 ．常用溶液配制················································································8 二、方法································································································8 (一) 细胞原代培养与传代培养······························································8 1．培养皿及器械的处理与准备·····························································8 2 ．胃癌细胞原代培养·········································································9 3 ．纯化与传代·················································································10 （二）Ming 氏浸润型胃癌细胞系的建立和特性······································10 1．细胞系的建立··············································································10