《DNA-PKcs影响肝癌耐药细胞BEL74025-FU耐药性的机制》.pdfVIP

第37卷第5期 遵义医学院学报 Vm．37No．5 2014年lO月 of Journal Medical Oct．2014 zunyl University 李长福，梁大敏，束 波，杨加伟，生 欣，范 芳 (遵义医学院生物化学教研室，贵州遵义563099) blot法检铡 转染干扰质粒后DNA—PKes基因的沉默效率；Western 定量PCR及Westernblot检测结果显示转染shDNA—PKcs后，DNA—PKcsmBNA及蛋白水平表达均下调(P0．05)； Western PKcs、Artemis蛋白的表达、但对P—Artemis的表达无影响。 [关键词]DNA依赖性蛋白激醇催化亚基；多药耐药；Artemis [中图法分类号】B735．7【文献标志码】A [文章编号】1000-2715(2014)05-0517-03 Tl璩effectofDNA—PKcssilenceonthe ofArtemisandP— gene expression inliver Artemis cancerBEL7402／5一FUcells Damin，Shu 厶醌口，咖，Liang Bo，y口愕Jiawei，Sheng渤，FanFang of Medical Guizhou 563099，China) (DepartmentBiochemistry，ZunyiUnivemity，Zunyi To observetheeffectofDNA—PKcs onthemechanismof resistance [Abstract]Objective genesilencing drug inBel7402／5一FURNAinterference DNA—PKcsinterferencew脑 by technology．MethodsTargeted plasmid transfectedintolivercancercellBEL7402／5一FU．DNA—PKcsWSdetectedReal—time silencingefl％iency by PCRandWesternblot．The ofArtemisandP—ArtemisWesternblot．ResultsReal—timePCR expression by andWesternblot indicatedthatthemRNAand levelsofDNA—·PKcs were assay protein expressiondownregulat-- of edaftertrandectionof blot showedthatthe Artemis shDNA—PKcs(P0．05)．Westernanalysis expression WaS decreasedon levels