《Effects of base analogues 5-bromouracil and 6-aminopurine on development of zebrafish Danio Rerio》.pdfVIP

Chinese Journal of Oceanology and Limnology Vol. 23 No. 1, P. 54-59, 2005 Effects of base analogues 5-bromouracil and 6-aminopurine on development of zebrafish Danio Rerio * † †,** †† SAWANT M. S. , ZHANG Shicui (张士璀) WANG Qingyin (王清印) † ( College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China) (††Yellow Sea Fisheries Research Institute, Qingdao 266071, China) Received Sept. 16, 2003; revision accepted Mar. 15, 2004 Abstract Zebrafish (Danio rerio) genetic screens allow isolation of a wide array of problems in vertebrate biology. The effects of base analogues 5-bromouracil and 6-aminopurine on the development of zebrafish embryos are reported for the first time in this study. The early development of the zebrafish embryos was little affected by 5-bromouracil and 6-aminopurine, while the late development (organogenesis) was significantly impaired. Embryos exposed to 5-bromouracil mainly showed curled tail, wavy body, golden pigmentation and the mouth with protruding lower jaw. 6-aminopurine-treated embryos had defective anterior structures, curled tails and wavy body. RAPD analysis showed that the majority of 5-bromouracil- and 6-aminopurine-treated larvae and fish shared banding patterns in common with the control, suggesting that most mutagenesis induced by these agents are point mutations. However, some fish derived from 5-bromouracil-treated embryos had golden (gol) pigmentation; and RAPD analysis revealed that their band patterns differed from those of the control. Possibly, 5-bromouracil can occasionally cause relatively extensive changes in the fish genom