《Laser photolysis of interaction of poly-guanylic acid (5′) with anthraquinone-2-sulfonate》.pdfVIP

Vol. 4 5 No. 4 SCIENCE IN CHINA (Series B) August 2002 Laser photolysis of interaction of poly-guanylic acid (5 ) with anthraquinone-2-sulfonate 1 2 2 MA Jianhua (马建华) , LIN Weizhen (林维真) , WANG Wenfeng (王文峰) , 2 2 2 HAN Zhenhui (韩镇辉) , YAO Side (姚思德) LIN Nianyun (林念芸) 1. College of Biology Engineering, Jimei University, Xiamen 361021, China; 2. Laboratory of Radiation Chemistry, Shanghai Institute of Nuclear Research, Chinese Academy of Sciences, Shanghai 201800, China Correspondence should be addressed to Ma Jianhua (email: jianhuam@263.net) Received November 7, 2001 Abstract The electron transfer reaction between triplet anthraquinone-2-sulfonate and poly- gua- nylic acid (5 ) in CH CN-H O (97 ︰3) has been investigated by 248 nm ( KrF ) laser flash pho- 3 2 tolysis. The transient absorption spectra and kinetics obtained from the interaction of triplet anthra- quinone-2-sulfonate and poly[G] demonstrate that the primary ionic radical pair, radical cation of poly[G] and radical anion of anthraquinone-2-sulfonate have been detected simultaneously. The free energy changes in the process of the electron transfer were also calculated. Keywords: anthraquinone-2-sulfonate, poly[G], laser photolysis . The photophysics and photochemistry of anthraquinone compounds have been studied exten- sively. Anthraquinone derivatives in particular that of anthraquinone-2-sulfonate (AQS) have been used as cleaving agent for duplex DNA[1 5] . However, direct observation of excited ion pairs of biomolecules especially the stabi