《PM2.5对大鼠心、肺、睾丸的氧化损伤作用》.pdfVIP

中国环境科学 2005,25(2) 160~164 China Environmental Science PM2.5 对大鼠心 肺 睾丸的氧化损伤作用 * 刘晓莉,李 红,孟紫强 (山西大学环境医学与毒理学研究所, 山西 太原 030006) 摘要 采用气管直接注入染毒法,研究 PM2.5 对大鼠心 肺和睾丸的氧化损伤作用.染毒后 24h 观察大鼠心 肺和睾丸抗氧化酶 (SOD,CAT,GSH-P ) 谷胱甘肽(GSH)和丙二醛(MDA)等指标的变化.结果表明,PM 染毒后,与对照组相比,肺抗氧化酶活性和脂质过氧化 X 2.5 (LPO)水平的变化最显著,出现明显的剂量-效应变化;睾丸仅高剂量组GSH-Px 酶活性和LPO 水平出现显著变化;心脏也仅是较高剂量组的 LPO 水平有显著升高,但各抗氧化酶活性变化无统计学意义.PM2.5 可对大鼠心 肺 睾丸具有不同程度的氧化损伤作用. 关键词 PM2.5 心 肺 睾丸 抗氧化酶 脂质过氧化 中图分类号 R994.6 文献标识码 A 文章编号 1000-6923(2005)02-0160-05 * Oxidative damage of PM2.5 on hearts, lungs and testicles of rats. LIU Xiao-li, LI Hong, MENG Zi-qiang (Institute of Environmental Medicine and Toxicology, Shanxi University, Taiyuan 030006, China). China Environmental Science, 2005,25(2) 160~164 Abstract The effect of oxidation damage of PM2.5 on hearts , lungs and testicles of rats was studied adopting the technique of toxic pollution with direct pouring through trachea. 24hours, after toxic pollution, the changes of indexes of antioxidative enzymes (SOD, CAT, GSH-Px) GSH and MDA in hearts, lungs and testicles of rats were observed. The influence of PM2.5 toxic pollution on antioxidative enzymes activities and lipid peroxidation (LPO) levels had difference in different organs. In lungs, the antioxidative enzyme activities and LPO levels changed most markedly with obvious dose-response changes comparing with the control group. In testicles, only the high dosage group of enzymes activities of GSH-P and LPO level change markedly. In hearts, also only LPO level of the higher dosage group raised markedly. But, X the changes of antioxidative enzymes activities were not significant. PM