复元活血汤对SD大鼠骨折早期血管内皮生长因子表达影响实验.pdfVIP

M ech a n ism o f E ffe et o f F u Y U a n H u o X u e D eco etio n o n T h e E x P r e ssio n o f Va scu la r E n d o th elia l G row th F a eto r d u r in g E a r ly F r a ctu r e H ea lin g M ajor :O rthoP aedies an d Traum atology ofT C M P ostgraduate : Z hang X iujie T u to r : X in J in A b str a C t A IM :TO ex P lo re th e effe ets o f fu yU an h u o x u e d eeo etio n o n th e ex P ressio n o f lo ea l v aseu lar en d oth elial growt h faetors (V E G F ) du rin g early fraeture h ealin g . M eth o d s :T h e an im a l m o d e ls o f oP en ed fra etu re w ith in tram edu llary fix atio n w ere estab lish ed in sixty m ale SD rats.T he 70 rats w ere div ided into herb grouP (fu yU- an hu o xue decoetion) an d saline grouP .Th e tissues in fraeture area w ere obtained at 3，5，7，14 and 2 ld ay s.S em i一q u antitativ e an a ly sis w a s u sed to d eteet th e ex P re ssio n o f V E G F th ro u g h R J .P C R m etho d . R esu lts : It in erea sed sign ifiean tly in V E G F at 3 d ay s. T h e ex P re ssio n o f V E G F w a s w eak er in ex P erim en tal grouP eom P ared w ith th e sa lin e grou P at 3 d ay s，wh ich w a s stro n g er in the h erb gro u P eom P ared w ith th e salin e gro u P at s ，7 ，14 an d Z I.T h e ex P re ssio n o f V E G F reaeh ed to th e P eak at 5 an d 7 d ay s . C o n clu sio n : F u y u an h u o x u e d eco etio n in early fra eture ean ae ee]erat e th e ex P re ssio n o f V E G F . K ey w o rd s Va scu lar en d o th elial eell growt h faetor : S D R ats ; F u Yu an H u o X u e d eco etio n