荧光假单胞菌GcM51A过氧化物还原酶和黑松的过氧化物酶的基因克隆及活性的研究.pdf

Abstract distributedin antioxidants biological Peroxiredoxins(Prxs)areenzymatic widely that constitutea ofheme-free reduce family peroxidases alkyl kingdoms，which of639 and this frame bp， paper,anopenreading hydroperoxideshydrogenperoxide．In fromPseudomonas whichencodeda of213aminoacid cloned protein residues，was GeM5—1Acarried woodnematode．Aminoacid jCluorescens bypine sequencealignment showedthattheencodedshareda of the 99％，97％and protein similarity 97％identity、Ⅳim antioxidantLsfAofP of thiol-specificprotein fluorescensQ2-87，theperoxiredoxin Pseudomonasand sp．GMl71-CysperoxiredoxinofPfluorescens ORFWasclonedinto vector 5btoconstructrecombinant expressingpET-1 plasmid in BL2 whichWasintroducedintoEscherichiacoli 5b一1一Cys—Prx 1(DE3)． pET-1 ofa27kDa WasachievedIPTGinduction．Therecombinant Overexpressionprotein by Was onaNipmatrix proteinpurifiedbyaffinitychromatography column．Non-reducing SDS—PAGEindicatedthat oftherecombinantindimerform． analysis part appeared resultsshowedthat recombinanthadboth and Bioassay purified protein peroxidase thioredoxin