白细胞介素-1在突出腰椎间盘中表达.docVIP

白细胞介素-1在突出腰椎间盘中的表达 中国脊柱脊髓杂志 2000年第1期第10卷 临床论著 作者：赵剑　赵敦炎　张明　姚登福　陆建新 单位：赵剑(南通医学院附属医院骨科)；赵敦炎(南通医学院附属医院骨科)；张明(南通医学院附属医院骨科)；姚登福(临床分子生物学中心　226001　江苏省献通市)；陆建新(临床分子生物学中心　226001　江苏省献通市) 关键词：白细胞介素-1；腰椎间盘突出症；坐骨神经痛 　　摘　要：目的：探讨白介素-1（IL-1）在腰椎间盘突出症坐骨神经痛发生中可能起到的作用。方法：对25例腰椎间盘突出症患者手术取出的髓核组织以酶联免疫吸附方法测定髓核中IL-1α和IL-1β。以5例因腰椎骨折手术患者术中所取髓核组织作为正常对照。此外，对患者坐骨神经疼痛程度以Dallas疼痛调查表作评分。结果：在髓核组织中测得IL-1α与IL-1β的存在。患者髓核中IL-1α含量明显高于正常组(P＜0.01)；而在不同突出类型之间IL-1α无显著性差异。IL-1α的含量与疼痛程度有相关性。结论：在腰椎间盘突出症坐骨神经痛的发生中，细胞因子尤其是IL-1α可能起到某种重要作用。 　　分类号：R681.5+3　　文献标识码：A 　　文章编号：1004-406X(2000)-01-0015-03 Presence of interleukin-1α in sciatic pain due to herniated disc of the lumbar spine ZHAO Jian,ZHAO Dun-yan,ZHANG Ming,et al 　　Abstract Objective：To determine the role of inflammatory cytokine interleukin-1(IL-1) in the mechanism of sciatic pain in lumbar disc herniation.Method：During operation,herniation lumbar discs of 25 patients were classified macroscopically into extrusion and sequestration types.Nucleus adjacent to nerve roots at the herniation were excised and analyzed by enzyme-linked immunosorbent assay for the presence of iL-1α and IL-1β.Another 5 normal nuclei from patients suffering from bursting fracture of lumbar spine were excised and analyzed by the same procedure.Dallas pain Questionnaire (DPQ) was recorded to evaluate the severity of pain.Result：IL-1α and IL-1β were detected in the homogenates of samples.IL-1α activity in herniated nucleus showed significant difference compared with normal nucleus and it showed significant correlation with the severity of pain evaluated by dPQ,while IL-1β did not.No significant difference of IL-1α activity were found between the different types of disc herniation.Conclusion：The results demonstrate that at the site of lumbar disc herniation,IL-1α and IL-1β are produced,especially IL-1α may play an important role in the mechanism of sciatic pain. 　　Author′s address Department of Orthopaedic Surgery,Affiliated hospital of Nantong Medical College,Nantong,Jiangsu,226001，China