低硒对发育期大鼠脑中CREB及核基质蛋白影响的研究.pdf

性细胞数表示 p-CREB 在大脑皮层（对照组＝171.89±63.062，低硒组＝109.44± 38.41，P0.01）海马（对照组＝28.14 ±9.25，低硒组＝8.67 ±1.53 P0.05）的改变， 显示低硒组较对照组均明显降低，且 p-CREB 在星形细胞中（对照组＝157.17± 44.80 ，低硒组＝29.40 ±13.11，P0.0 1）较胶质细胞（对照组＝68.80 ±13.78，低 硒组＝51.50 ±14.86，P0.05 ）下降明显（分别下降 81.3 ％，25.1 ％）。利用高效 毛细管电泳 CZE 分离模式能够将 NMPs 分离出 3~4 个蛋白峰，波形较稳定，进 样 9min 左右有一个蛋白峰低硒组明显低于对照组。 结论 （1）低硒膳食能够引起发育期大鼠大脑皮层和海马的氧化代谢水平降低。 （2 ）CREB 表达减少反映低硒能够引起大脑皮层和海马神经元功能下降，提示 低硒可能导致学习记忆能力降低的潜在因素之一。（3 ）低硒能够引起脑 NMPs 的变化。 关键词 硒 脑 cAMP 反应元件结合蛋白 核基质蛋白 高效毛细管电泳 万方数据 The Effects of Selenium Deficiency Diet on CREB and Nuclear Matrix Proteins in Brain of Developing Rat Postgraduate: Geng Yiqun Supervisor: Prof.Xu Xiaohu Prof.Su Min Abstract Object To observe the changes of lipid peroxide in the cortex and hippocampus of developing rat induced by selenium deficiency diet and study the neuron function by the content and activity detection of cAMP Response Element-Binding Protein (CREB) in cortex and hippocampus. To study the alteration of NMPs components. Material and method Groups of healthy adult SD rats with equal male and female at random were fed with selenium deficiency and selenium adequate food. The third generation at 21 days of age were killed and the cortex and hippocampus were dissected on ice rapidly. The contents of MDA were assayed by 2-thiobarbituric acid (TBA) test. The expression of CREB and p-CREB were examined by western blot in the extracted total protein from cortex and hippocampus. The expression and the location of CREB and p