模板法构筑纳米金复合材料和其在检测中的应用.pdf

摘 要 曼散射（SERS）基底的活性。原本对SERS信号响应较弱的10 nm直径的金纳米颗 粒，在自组装到DNA上形成具有纳米间隙的金纳米颗粒网络后，产生了均匀、一 致、强烈的SERS增强响应。此方法制备SERS基底产率高、SERS增强效果好， SERS信号稳定性和重复性相对常规孤立的金纳米颗粒SERS基底有很大提高。 关键词: 模板 免疫传感器 多壁碳纳米管 循环伏安法 SERS III ABSTRACT ABSTRACT With the development of science and technology, more and more materials are taken into research by scientists ，from macro-materials to nano-materials, from a single material to composite materials. At the same time, the preparation of materials also changed a lot. As a new method, template has become more and more important in the detection field for the ability to synthesize composite materials with particular characters. In this paper we firstly synthesized composite materials based on gold nanoparticles, and then studied the application of the materials in the detection of R6G and Salmonella. The research focused on the 863 program in our lab carried out in this paper can be devided into three aspects: The first part is the construction of chemical resistance immunosensor based on the gold-multi-walled carbon nanotubes composites materials to the detection of Salmonella flagellin. Firstly, muti-functional processing of template-carbon nanotubes has been carried out to form a series of active sites. The functional steps are oxidation, thiolation and then the freshly synthesized Au nanoparticles with the dimension of 15nm were adhered to the active sites of multi-walled carbon nanotubes to prepare composite materials ，we finally use this materials to prepare the immunoelectrode to achieve label-free detection of the Salmonella enteritidis. The results showed that the modified electrode has excellent electrical respo