材料腐蚀微生物腐蚀..ppt

* Influence of a dual-species biofilm on the corrosion of mild steel 报告时间：2014.10.14 Contents Abstract Introduction Experimental approach Conclusion Results 1.Abstract Two bacterial species: the iron-reducer, Shewanella oneidensis (MR-1), and the sulfate-reducer, Desulfovibrio desulfuricans (G20). Electrochemical impedance spectroscopy (EIS) shows that over a short time period, co-cultures of MR-1 and G20 protect steel from corrosion. its potential applicability to the design of biological corrosion-control measures. 2.Introduction MR-1 can couple the oxidation of lactate to the reduction of poorly crystalline ferric (hydr)oxides [Fe(OH)3]: 1/4CH3CHOHCOO-+Fe(OH)3 +2H+ 1/4CH3COO-+ Fe2+ + 1/4CO2(g) +11/4H2O whereas G20 can couple the oxidation of lactate to the reduction of sulfate: 2CH3CHOHCOO- +SO42-+2H+ 2CH3COO- +2CO2(g)+ HS-+ 2H2O 3.Experimental approach 1.菌种来源 杀菌 浸入 洗净、挂擦 富集培养 经初步分子生物学分析, 该富集细菌中存在IRB和SRB。将培养2 周的混合细菌按10% 体积比接种到试验体系中, 进行相关试验。进一步使用琼脂板固体培养基对富集细菌进行分离纯化。 2.对腐蚀产物进行扫描电镜与能谱分析 Bacterial biofilms were imaged by environmental scanning electroscopy (ESEM) using a Philips XL30 ESEM-FEG. Samples were viewed using temperature and hydration control provided by a water-cooled Peltier specimen stage. The sample chamber water vapor pressure was 5.0 Torr at a 3.4 ℃. Samples were viewed using an accelerating voltage of 20 keV and a beam spot size of approximately 10 nm at the specimen with a working distance of 10 mm. 3.电化学实验（三电极体系、EIS、Nyquist、Bode) Stainless steel 316 SCE 实验分组： single-species experiments dual-species experiments MR-1 G20 Nothing MR-1 MR-1：G20=10：1 MR-1：G20=100：1 Electrochemical impedance spectroscopy (EIS) was used to determine how the bacteria influenced the corrosion potential (Ecorr) and corrosion rate of the steel.Impedance spectra were recorded with an applied alternating current signal of 10 mV (rms) and a frequency range from 105 to 10-4 Hz using Gamry equipment. EIS In systems with a single relaxation time, Nyquis