五苓散对高盐诱羔羊尿结石防治作用的研究.pdf

摘 要 本试验采用逐步提高日粮中磷和镁含量的方法诱发雄性羔羊产生尿结石，同时观 测五苓散对高磷和高镁诱发羔羊尿结石的防治效果。 试验中通过观察试验羊的症状、解剖查看泌尿系统结石产生情况对诱发尿结石及 五苓散对其的防治效果进行判定。通过尿沉渣的观察和膀胱结石成分的分析，并根据 数据软件对血液和尿液中电解质和肾功能指标的分析结果，探讨血液和尿液中电解质 和肾功能指标与高磷和高镁诱发羊尿结石之间的联系。研究结果表明： 1.饲喂含高磷和高镁日粮成功的诱发了羔羊的尿结石。X 射线荧光光谱仪分析结 果显示膀胱结石含有的主要元素为镁和磷，且尿沉渣的晶型也与鸟粪石的晶型相符。 尿结石发生过程中血液中的磷、镁、尿素、肌酐升高明显。尿液中镁和磷先升后降， 尿素、肌酐降低，尿蛋白升高。这说明高镁和高磷日粮首先引起血液中的磷和镁含量 的升高，继而引起尿液中磷和镁含量升高，进而尿液中磷和镁析出形成尿结石，且尿 结石发生过程中羊的肾脏功能受到了严重的损害。 2.五苓散对高磷和高镁日粮诱发的羔羊尿结石预防效果显著并有一定的治疗效 果。预防组羊血液中的磷、尿素、肌酐均维持正常水平，镁高于对照组但显著低于诱 石组；尿液中磷的升高和尿素、肌酐降低均不显著，镁高于对照组但显著低于诱石组 最大值，尿蛋白在中后期显著低于对照组。治疗组中血液中磷、镁很快降到正常水平， 而尿素和肌酐则缓慢降低；尿液中镁和磷先升高后降低，肌酐和尿素缓慢升高。这说 明五苓散可以抑制高镁和高磷诱发羔羊尿结石过程中对肾脏的损害并维持其正常的 功能，而且对于高镁和高磷诱发羔羊尿结石后受损的肾功能有一定的修复作用，能够 加强血液中过高浓度镁和磷的排泄，降低尿液中晶体的析出。 关键词：高盐；羔羊；尿结石；五苓散；防治 Preventive and Therapeutic Effect of Wu Ling San on High Salt Induced Lamb Urinary Calculi Abstract The urinary calculi of male lamb were induced by gradually increasing content of phosphorus and magnesium in the daily ration and the preventive and therapeutic effect of Wu Ling San on Lamb induced Urinary Calculi were observed afterwards in this experiment. Induced stones and the therapeutic effects were determined by the observation on symptoms of experimental sheep and the stone formation in urinary tracts. The correlations between sheep urinary Calculi and blood, urine electrolyte; and the index for renal function were defined through the observation on urine sediments and the component analysis on bladder stones, and the determination of sodium, potassium, chlorine, calcium, magnesium, inorganic phosphorous, ammonia, urea nitrogen, creatinine contents in both serum and urine; total protein content in serum and urine protein content; and the