Ac_Ds转座子激活标签技术研究进展.pdfVIP

中国农业科学 2013,46(14):2845-2855 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2013.14.001 Ac/Ds 转座子激活标签技术研究进展 王文静，马浩然，李加纳，张洪博 （西南大学农学与生物科技学院/重庆油菜工程技术中心/南方山地农业教育部工程研究中心，重庆 400716 ） 摘要：随着植物基因组测序工作的迅速发展，植物功能基因组学已经成为植物分子生物学研究的重要内容， 而大量有表型的突变体是进行功能基因组学研究的重要基础。Ac/Ds 转座子激活标签技术（Ac/Ds transposon activation tagging system）的产生使Ac/Ds 转座子标签技术具有了产生显性突变的功能，并可避免在突变体库 创建过程中进行大量繁重的人工转基因操作，是比较理想的植物功能基因组学研究工具。本文将对Ac/Ds 转座子 激活标签技术在发展过程中利用不同激活标签创建植物突变体库的研究进行介绍，并对近二十几年来报道的相关 应用研究展开论述，以期对该领域的技术进步和应用研究提供一些有益的启发和参考。 关键词：Ac/Ds 转座子激活标签技术；突变体库；功能基因组学 Research Progresses in Ac/Ds Transposon Activation Tagging System WANG Wen-jing, MA Hao -ran, LI Jia-na, ZHANG Hong-bo （College of Agronomy and Biotechnology, Southwest University/ Chongqing Rapeseed Engineering Research Center/ Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 4 00716） Abstract: Along with the development of plant functional genomics, creation of an informative mutant population is becoming more and more important. The Ac/Ds transposon system is an effective tool for mutant creation, especially for plant species with difficulties in transformation. And, the development of Ac/Ds transposon activation tagging system makes it feasible to create dominant mutations. In this paper, the progresses in the development of Ac/Ds transposon activation tagging system with versatile activation tagging were introduced and the future perspectives of this system in creation of plant mutant populations were discussed. Key words: Ac/Ds transposon activation tagging system; mutation population; functional genomics 手段。目前，创建植物突变体库的方法主要有理化诱 0 引言