IBA与GA3调控百合鳞片扦插繁殖的“淀粉-蔗糖”代谢机制.pdfVIP

中国农业科学 2011,44(4):798-806 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2011.04.018 IBA与GA 调控百合鳞片扦插繁殖的“淀粉-蔗糖”代谢机制 3 孙红梅，何 玲，王微微，贾子坤，李天来 (沈阳农业大学园艺学院/设施园艺省部共建教育部重点实验室/辽宁省设施园艺重点实验室, 沈阳110161) 摘要：【目的】研究 IBA 与 GA 3 促进百合鳞片扦插繁殖的碳水化合物代谢机制，为调控鳞片繁殖技术、深入 研究小鳞茎形成发育的生理机制奠定基础。【方法】以亚洲系‘精粹’百合为试材，设计 200 mg·L-1 IBA 浸泡鳞片 2 h 和 200 mg·L-1 GA 浸泡鳞片 8 h 两个处理，以同体积清水浸泡鳞片 2 h 为对照，测定母鳞片不同部位和小鳞茎 3 中淀粉、蔗糖含量及相关酶活性。【结果】施用 IBA 与 GA促进了母鳞片的淀粉降解、蔗糖合成以及鳞片上、中部 3 的糖类物质向基部的运转，加快了小鳞茎的形态建成。母鳞片的淀粉降解部分受淀粉磷酸化酶（starch phosphorylase, SP）调控，蔗糖含量与蔗糖合成酶 （sucrose synthase, SS）、蔗糖磷酸合成酶 （sucrose phosphate synthase, SPS）活性极显著正相关。IBA促进鳞片淀粉降解、可溶性糖利用或运转的效用高于GA3,且IBA有利于 母鳞片蔗糖的尽早合成；GA3 在培养前期抑制了鳞片上部和中部的淀粉降解，并促进母鳞片合成较多的蔗糖。【结 论】IBA和GA3 促使母鳞片内淀粉以及蔗糖的代谢，相关酶活性提高，从而提高繁殖系数或获得最佳小鳞茎个体。 关键词：百合；鳞片扦插；淀粉；蔗糖 Mechanism of Starch-Sucrose Metabolism Regulated by IBA as Well as GA3 During Scale Cutting Propagation in Lilium SUN Hong-mei, HE Ling, WANG Wei-wei, JIA Zi-kun, LI Tian-lai (College of Horticulture, Shenyang Agricultural University/Key Laboratory of Protected Horticulture, Ministry of Education/Key Laboratory of Protected Horticulture of Liaoning Province, Shenyang 110161) Abstract:【Objective 】 The effects of IBA and GA3 on starch-sucrose metabolism during scale cutting propagation were studied with the aim to provide a theoretical and practical basis for regulating the development of bulblets and explore the physiological mechanism of scale propagation. 【Method 】The scales of Lilium Asiatic hybrids ‘Elite’ were immersed in IBA solution