超高压对大肠杆菌细胞膜流动性的影响.pdfVIP

中国农业科学 2009,42(4):1365-1371 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2009.04.029 超高压对大肠杆菌细胞膜流动性的影响 高瑀珑, 鞠兴荣，邱伟芬，吴 定 （南京财经大学食品科学与工程学院，南京 210003 ） 摘要：【目的】对超高压作用后大肠杆菌细胞膜的荧光偏振度和微黏度的影响进行研究，探讨超高压对大肠 杆菌细胞膜流动性的影响。【方法】以1,6-二苯基-1,3,5,-己三烯（DPH）为荧光探针标记大肠杆菌细胞膜，用荧 光偏技术测定大肠杆菌细胞膜经超高压作用后荧光偏振度和微黏度的变化。【结果】建立了用DPH 标记和荧光偏振 法测定大肠杆菌细胞膜流动性条件；不同的处理压力和时间对大肠杆菌细胞膜的荧光偏振度和微黏度的影响不同， 350～400 MPa 作用 15～40 min，细胞膜荧光偏振度及微黏度达到稳定水平，350 MPa 作用 15 min 已经使细胞膜 流动性显著降低(P0.05)。【结论】随着压力的增大和保压时间的延长，大肠杆菌细胞膜荧光偏振度及微黏度增大， 流动性降低，大肠杆菌死亡数增加。当压力和保压时间增加到一定程度(350 MPa, 15 min)，荧光偏振度及微黏度 达到相对稳定状态，大肠杆菌几乎全部死亡，说明在超高压的作用下大肠杆菌细胞膜荧光偏振度和微黏度的变化 与死亡相关性好(P ＜0.05)，这为超高压灭活大肠杆菌提供了理论证据。 关键词：超高压；大肠杆菌；细胞膜；流动性 Effects of High Hydrostatic Pressure on Membrane Fluidity of Escherichia coli GAO Yu-long, JU Xing-rong, QIU Wei-fen, WU Ding (College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210003) Abstract: 【Objective 】 Effects of high hydrostatic pressure (HHP) on the fluorescence polarization and microviscidity of Escherichia coli membrane were investigated, and the effect of HHP on the fluidity of E. coli membrane was explored. 【Method 】 In the present study, 1,6-diphenyl-1,3,5-hexatriene (DPH) was used as fluorescence probe to label the membrane of E. coli, and to determine the changes of fluorescence polarization and microviscidity of E. coli membrane by fluorescence polarization technique after HHP treatments. 【Result 】 The conditions were established to measure the membrane fluidity of E. coli membrane by labeling with DPH and the method of fluorescence polarization. The experimental results also indicated that