灰葡萄孢BC7-3菌株固体发酵条件的优化.pdfVIP

中国农业科学 2011,44(16):3477-3484 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2011.16.022 灰葡萄孢BC7-3 菌株固体发酵条件的优化 张利辉，冯江兰，董金皋 （河北农业大学生命科学学院真菌毒素与植物分子病理学实验室，河北保定 07100 1） 摘要：【目的】探讨灰葡萄孢BC7-3 菌株固体发酵的基本条件以提高其除草活性物质的产量。【方法】通过正 交试验设计确定最优化的培养基组合，并结合生长抑制法测定外加碳源、氮源等因素对除草活性物质产生的影响， 以确定最佳的发酵条件。【结果】确定了小米5 g、高粱5 g、麦麸5 g、玉米秸秆2.5 g 为最佳复合培养基质； 明确了该菌株在加入1%硝酸铵、1%蔗糖的培养基中，20℃黑暗条件下，接种量8%、含水量80%、静置培养15 d， 得到的除草活性物质活性最强。【结论】固体发酵适合于灰葡萄孢代谢产物中除草活性物质的产生。 关键词：灰葡萄孢；固体发酵；除草活性；黄顶菊 Optimization of Solid Fermentation of Botrytis cinerea BC7-3 Strain ZHANG Li-hui, FENG Jiang-lan, DONG Jin-gao (Labatory of Mycotoxin and Molecular Plant Pathology, College of Life Science, Agricultural University of Hebei, Baoding 071001, Hebei) Abstract: 【Objective 】 In order to enhance the production of the herbicidal substances and to furthermore separate the crude toxin, the solid fermenting conditions of BC7-3 were researched in an experiment. 【Method 】 BC7-3 was fermented with solid media by using a method of orthogonal combination and different factors affecting toxin-producing was researched. The crude toxin was obtained by extracting with acetone and the herbicidal activity of crude toxin to Flanueria bidentis was bioassayed by using the method of growth inhibition. 【Result 】The medium containing millet at 5 g, sorghum at 5 g, wheat bran at 5 g and corn straw at 2.5 g were found to be the most optimum. The herbicidal activity of the crude toxin reached the strongest when BC7-3 was inoculated with the inoculum concentration of 8% in the optimum medium containing 1% sucrose, 1% ammonium mitrate and 80% water and fermented in the darkness for 15 days under the condition of 20 ℃. 【Conclusion 】 It is suggested that solid fermentation is suitable for the