基于PCR技术的玉米丝轴黑粉菌侵染率.pdfVIP

中国农业科学 2006,39(9):1804-1809 Scientia Agricultura Sinica 基于PCR技术的玉米丝轴黑粉菌侵染率及扩展进程的研究 1 1 2 2 1 1 倪 深 ，肖炎农 ，王凤格 ，赵久然 ，张绍鹏 ，郑用琏 1 2 （华中农业大学作物遗传改良国家重点实验室／国家分子技术育种中心，武汉 430070 ； 北京市农林科学院玉米研究中心, 北京 100089） 摘要：【目的】以对玉米丝黑穗病高感的黄早四和抗病的Mo17自交系为材料，用丝轴黑粉菌进行接种，研究 病菌对玉米的侵染率及扩展进程。【方法】采用丝黑穗病病原菌基因组特异引物的PCR技术，分别对幼苗期的根、 叶和成熟期的根、茎、叶、雄穗生长锥DNA 进行PCR扩增。【结果】特异引物的PCR技术不仅能够在早期准确鉴定 玉米幼苗是否受到病菌的侵染，而且可以跟踪丝轴黑粉菌在体内扩展进程。【结论】玉米对丝黑穗病的抗性差异主 要表现为抗侵染和抗扩展，抽穗期雄穗对病樱的形成不能表现出明显的抗性效应。 关键词：玉米；丝黑穗；PCR；侵染；扩展 The Research of Sporisorium reiliana’s Infection Efficiency and Spreading in Maize Based on PCR Assays 1 1 2 2 1 1 NI Shen , XIAO Yan-nong , WANG Feng-ge , ZHAO Jiu-ran , ZHANG Shao-peng , ZHENG Yong-lian 1 （National Key Laboratory of Crop Genetic Improvement ／National Center of Crop Molecular Breeding, Huazhong Agricultural University, Wuhan 430070; 2 Maize Research Center, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100089 ） Abstract: 【Objective 】 Head smut of maize substantially reduces grain yield. Here we demonstrate a recent survey of the rate of infection of head smut by PCR. 【Method 】Using gene-specific primer derived from the head smut genomics and maize temporal and tissue-specific DNA, the invading process of the pathogen is displayed clearly. 【Result 】The study shows that the differential resistance of maize against head smut depends mainly on the ability of resistance against infection and spreading. 【Conclusion 】The above effect disappears in the stamen when the tumor forms during tasseling stage. Key words: Maize; Head smut; PCR; Infection; Spread 的玉米幼苗进行侵