两种水稻OsRhoGDIs基因启动子的克隆及分析.pdfVIP

中国农业科学 2008,41(10):2916-2922 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2008.10.002 两种水稻 OsRhoGDIs 基因启动子的克隆及分析 张利娟，梁卫红，刘悦霞，刘肖飞，侯成千，毕佳佳 （河南师范大学生命科学学院，河南新乡 453007 ） 摘要： 【目的】OsRacD 属于水稻小 GTP 结合蛋白 Rho 家族,是与光敏核不育水稻光周期育性转换相关的关键 基因,功能之一是通过控制花粉管的延伸生长影响水稻的育性。在采用酵母双杂交技术,筛选到与OsRacD相互作用 的两种鸟苷酸解离抑制因子的编码基因 OsRhoGDI1 和 OsRhoGDI2 的基础上，为深入分析 OsRhoGDIs 的调控机制以 及与 OsRacD 的关系，对其上游调控序列进行克隆和分析。【方法】采用 PCR 方法克隆了OsRhoGDI1 和 OsRhoGDI2 的上游调控序列，并利用网络工具对启动子顺式作用元件进行预测。【结果】两种 OsRhoGDIs 基因具有与激素应答、 光调控及胁迫诱导应答相关元件,且一些元件相同或相似；OsRhoGDIs 与 OsRacD 启动子元件的比较显示，都具有 与花粉管萌发相关的多个元件。【结论】OsRhoGDIs 的表达调控方式复杂，可能受多条信号通路的共同调控；同 时也提示 OsRhoGDIs 与 OsRacD 基因可能通过协同表达控制光敏核不育水稻的育性。 关键词：水稻；OsRhoGDI；Rho；OsRacD；顺式作用元件 Cloning and Analysis of the Promoters of Two OsRhoGDIs Genes in Rice ZHANG Li-juan, LIANG Wei-hong, LIU Yue-xia, LIU Xiao-fei, HOU Cheng-qian, BI Jia-jia (College of Life Science, Henan Normal University, Xinxiang 453007, Henan) Abstract: 【Objective 】OsRacD belonging to rice (Oryza sativa L. ssp. japonica) Rho family of the small GTPases, is a pivotal gene involved in rice photoperiod fertility conversion of photoperiod sensitive genic male sterile rice , which influences rice fertility via controlling the pollen tube growth. Using OsRacD as bait, two GDP dissociation inhibitor genes designated as OsRhoGDI1 and OsRhoGDI2 were screened by yeast two-hybrid system. 【Method 】To further study the regulation mechanism of OsRhoGDIs ,and also the relationships between OsRhoGDIs and OsRacD, the upstream regulation sequences of OsRhoGDI1 and OsRhoGDI2 were cloned by PCR, the cis-elements in the promoters were perdicted with internet tools in this study. 【Result 】The Results showed that there were sever