响应面优化苹果皮渣多酚超声提取工艺研究.pdfVIP

中国农业科学 2013,46(21):4569-4577 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2013.21.020 响应面优化苹果皮渣多酚超声提取工艺研究 李 珍，哈益明，李 安，李伟明，王 锋，李庆鹏 （中国农业科学院农产品加工研究所/农业部农产品加工与质量控制重点实验室，北京 100193） 摘要：【目的】研究超声波辅助乙醇提取苹果皮渣多酚的最佳工艺条件。【方法】以工厂榨汁后的苹果皮渣为 原料，在单因素试验的基础上，选取提取时间、超声功率、提取温度、料液比为自变量，多酚的提取率为响应值， 根据响应面Box-Benhnken 试验设计原理，采用四因子三水平的分析法模拟得到二次多项式回归方程的预测模型， 优化苹果皮渣多酚超声提取工艺。【结果】回归模型具有高度显著性，方程对试验拟合较好，可以对苹果皮渣多酚 得率进行很好的分析和预测；各因子对提取率的影响大小依次是提取温度＞料液比＞提取功率＞提取时间；响应 面分析图表明提取时间和超声功率交互作用不显著，提取温度和超声功率交互作用极显著，料液比的主效应大于 温度；超声波辅助乙醇提取苹果皮渣的最佳工艺条件为超声时间10 min，提取温度65℃，超声功率503 W，料液 -1 -1 比1 ﹕30。【结论】多酚的提取率达4.53 mg·g ，与预测值4.55 mg·g 基本一致。 关键词：苹果皮渣; 多酚；超声波；响应面 Optimization of Ultrasound-Assisted Extraction Technology of Polyphenols from Apple Pomace by Response Surface Methodology LI Zhen, HA Yi-ming, LI An, LI Wei-ming, WANG Feng, LI Qing-peng (Institute of Agro-food Science Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-product Process ing, Ministry of Agriculture, Beijing 100193) Abstract: 【Objective 】The objective of this study is to optimize ultrasound-assisted ethanol extraction of polyphenols from apple pomace. 【Method 】Residues from juice extraction of apple were used in this paper. On the basis of single-factor test, the response surface methodology was used to investigate the effects of processing parameters, including extraction time, temperature, ultrasound power and solvent/material ratio. The extraction rate of polyphenols was chosen as response value, mathematical mo del was established by implementing four factors and three levels. 【Result 】 The model w