西芹浸提液对黄枯萎病菌化感作用机理的研究——西芹鲜根及根际物浸提液处理后黄瓜叶片内酶活性的变化.pdfVIP

摘 要 本实验在黄瓜幼苗长到第 1 片真叶横宽 5cm 大小时，分别用西芹鲜根和根际区 物的三种浸提液进行化感处理 (灌根处理)，灌根前 1d 和最后 1 次灌根后 1d、5d、 10d、15d、20d 随机取黄瓜同叶位的真叶来作为供试材料，对黄瓜叶片防御酶系、 β-1,3-葡聚糖酶、几丁质酶、吲哚乙酸氧化酶、细胞壁降解酶活性进行测定，主 要结果如下： (1) 经过西芹鲜根和根际区物不同溶剂的浸提液灌根后，与对照空白溶剂比较 可以显著提高黄瓜叶片内的防御酶系的活性。 (2) 黄瓜幼苗叶片内β-1,3-葡聚糖 酶和几丁质酶的活性在西芹鲜根和根际区物不同溶剂的浸提液灌根后都显著高于 对照溶剂。(3) 经过西芹鲜根和根际区不同溶剂的浸提液处理后，可以显著减缓了 黄瓜幼苗叶片内的 IAA 氧化酶活性的增强。(4) 经过西芹鲜根和根际区不同溶剂的 浸提液处理后，可以显著降低了黄瓜幼苗叶片内的羧甲基纤维素酶和β-葡萄糖苷 酶活性。且各指标的变化幅度均是丙酮浸提液处理大于乙醇浸提液处理，乙醇浸提 液处理大于蒸馏水浸提液处理、鲜根浸提液的处理大于根际区物浸提液。 说明经过西芹三种浸提液灌根处理后会显著增加黄瓜抗枯萎病能力，进一步阐 述了西芹浸提液对黄瓜枯萎病菌化感作用的机理。 关键词：西芹浸提液；防御酶；黄瓜枯萎病；β-1,3-葡聚糖酶活性；几丁质酶活性； 吲哚乙酸氧化酶；细胞壁降解酶 The study on the allelopathy mechanism of the extract of celery to the fusarium wilt of cucumber — The variation of the enzyme activities in cucumbers leaves after the treatment with the extract of fresh root and rhizosphere area of celery Abstract In this study ,we treat the cucumber with the leach liquor collected from the fresh root and rhizosperic substance by distilled water 、ethylalcohol and acetonum and determine the activity changement of the defensive enzyme 、chitinase、Beta – 1,3 – glucanase、indoleacetic acid oxidase 、cell wall-degrading enzymes in the leaves, aiming at clarifying the allelopathy mechanism of the fresh root of celery and rhizosperic substance,We began to do the root irrigation treatment with the three leach liquor collected from the fresh root of celery and rhizosperic substance when the cucumber seedlings’ first main leaf grow to a certain size.On one day before the treatment and one ,five,ten,fifteen and twenty days after the last irrigation, we took the leaf at the