Microbial community structure and diversity in deep-sea hydrothermal vent sediments along the Eastern Lau Spreading Centre.pdfVIP
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ActaOceano1.Sin.,2013,Vo1.32,No.2,P42—51
DOI:10.1007/s13131—013—0276—6
http://www.hyxb.org.cn
E—mail:hyxbe@263.net
Microbialcommunitystructureanddiversiyt indeep-sea
hydrothermalventsedimentsalongtheEasternLau
SpreadingCentre
WEIManman ,ZHANGRubing。,WANGYuguang,JIHouguo,ZHENGJia,
CHENXinhua4.ZHOUHongbol,
SchoolofM ineralsProcessingandBi0engineering,CentralSouthUniversity,Changsha410083,China
KeyLaboratoryofBiometallurgy,Ministry ofEducation,Changsha410083,China
。QingdaoInstituteofBioenergyandBioprocessTechnology,ChineseAcademyofSciences,
Qingdao266101,China
ThirdInstituteofOceanographyState,OceanicAdministration,Xiamen361005,China
Received19May2011;accepted24April2012
@TheChineseSocietyofOceanographyandSpringer—VerlagBerlinHeidelberg2013
Abstract
Theaim ofhtisstudyistoinvestigatem icrobialstructuresanddiversitiesinfiveactivehydrothermalfields’
sedimentsalongtheEasternLauSpreadingCentre(ELSC)inhteLauBasin (southwestPacific).Microbial
communitiesweresurveyedbydenaturedgradientgele1ectr0Dh0resis(DGGE)andclone1ibraryanalysis
of16SrRNAgenes.Thedifierencesinmicrobial communitystructuresamongsedimentsamplesfrom the
fivedeep—seahydrothermalsiteswererevealedbyDGGEprofiles.ClusteranalysisofDGGEprofilessepa—
ratedthefivehydrohtermal samplesintotwogroups.Fourdifferent16SrRNAgeneclonelibraries.repre—
sentingwtoselectedhydrothermalsamples(19—4Ⅳ G8and19—4Ⅳ Gl1),wereconstructed.Twenyt—htree
and32phylotypeswereidentifiedfrom 166and160bacterialclonesrespectively,includingProteobacteria,
Bacteroidetes,Firmicutes,NitrospiraeandPlanctomycetes.Thephylum Proteobacter isdominantinboth
bacterial librarieswithapredominanceofGma ma—Proteobacteria.A totalof31and25phylotypeswere
obtainedrfom 160and130archaeal clonesrespectively,includingMiscellaneousCrenarchaeoticGroup,
MarineGroupIandIll,MarineBenthicGroupE.TerrestrialHotSpringCrenarchaeotaandDeep。seaHy-
drothermalVentEuryarchaeota.Theseresultsshow avarieyt ofclonesrelatedtothoseinvolvedinsulfur
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