Microbial community structure and diversity in deep-sea hydrothermal vent sediments along the Eastern Lau Spreading Centre.pdfVIP

Microbial community structure and diversity in deep-sea hydrothermal vent sediments along the Eastern Lau Spreading Centre.pdf

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ActaOceano1.Sin.,2013,Vo1.32,No.2,P42—51 DOI:10.1007/s13131—013—0276—6 http://www.hyxb.org.cn E—mail:hyxbe@263.net Microbialcommunitystructureanddiversiyt indeep-sea hydrothermalventsedimentsalongtheEasternLau SpreadingCentre WEIManman ,ZHANGRubing。,WANGYuguang,JIHouguo,ZHENGJia, CHENXinhua4.ZHOUHongbol, SchoolofM ineralsProcessingandBi0engineering,CentralSouthUniversity,Changsha410083,China KeyLaboratoryofBiometallurgy,Ministry ofEducation,Changsha410083,China 。QingdaoInstituteofBioenergyandBioprocessTechnology,ChineseAcademyofSciences, Qingdao266101,China ThirdInstituteofOceanographyState,OceanicAdministration,Xiamen361005,China Received19May2011;accepted24April2012 @TheChineseSocietyofOceanographyandSpringer—VerlagBerlinHeidelberg2013 Abstract Theaim ofhtisstudyistoinvestigatem icrobialstructuresanddiversitiesinfiveactivehydrothermalfields’ sedimentsalongtheEasternLauSpreadingCentre(ELSC)inhteLauBasin (southwestPacific).Microbial communitiesweresurveyedbydenaturedgradientgele1ectr0Dh0resis(DGGE)andclone1ibraryanalysis of16SrRNAgenes.Thedifierencesinmicrobial communitystructuresamongsedimentsamplesfrom the fivedeep—seahydrothermalsiteswererevealedbyDGGEprofiles.ClusteranalysisofDGGEprofilessepa— ratedthefivehydrohtermal samplesintotwogroups.Fourdifferent16SrRNAgeneclonelibraries.repre— sentingwtoselectedhydrothermalsamples(19—4Ⅳ G8and19—4Ⅳ Gl1),wereconstructed.Twenyt—htree and32phylotypeswereidentifiedfrom 166and160bacterialclonesrespectively,includingProteobacteria, Bacteroidetes,Firmicutes,NitrospiraeandPlanctomycetes.Thephylum Proteobacter isdominantinboth bacterial librarieswithapredominanceofGma ma—Proteobacteria.A totalof31and25phylotypeswere obtainedrfom 160and130archaeal clonesrespectively,includingMiscellaneousCrenarchaeoticGroup, MarineGroupIandIll,MarineBenthicGroupE.TerrestrialHotSpringCrenarchaeotaandDeep。seaHy- drothermalVentEuryarchaeota.Theseresultsshow avarieyt ofclonesrelatedtothoseinvolvedinsulfur

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