栽培萝卜种质亲缘关系的AFLP分析.pdfVIP

中国农业科学 2005,38(5):1017-1023 Scientia Agricultura Sinica 栽培萝卜种质亲缘关系的AFLP分析 1, 2 1 2 1 1 孔秋生 ，李锡香 ，向长萍 ，邱 杨 ，沈 镝 1 2 ( 中国农业科学院蔬菜花卉研究所，北京 100081 ； 华中农业大学园艺林学学院，武汉 430070 ） 摘要：利用筛选出的8对引物对56份来源于不同国家和地区的栽培萝卜种质的亲缘关系进行了AFLP分析， 共扩增出 327 条带，其中多态性带 128 条，多态性位点百分率 39.1%，显示出栽培萝卜种质之间存在着较丰富的 遗传多样性。系统聚类分析将供试材料分为 5 类 9 组，主坐标分析将其分为 4 类 7 组，2 种分类方法所获结果基 本一致。基于分子标记的分类与种质的表型基本吻合。 关键词：萝卜；Raphanus sativus L.；AFLP；聚类分析；主坐标分析；亲缘关系 Phylogenetic Analysis of Cultivated Radish (Raphanus sativus L.) Germplasm with AFLP 1, 2 1 2 1 1 KONG Qiu-sheng , LI Xi-xiang , XIANG Chang-ping , QIU Yang ; SHEN Di 1 2 （Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081; College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070 ） Abstract: Phylogenesis of 56 cultivated radish germplasm from different countries or regions was analyzed with amplified fragment length polymorphism (AFLP). A total of 327 bands were detected with 8 pairs of informative AFLP primers among the accessions tested. Of them, 128 bands (39.1%) were polymorphic, indicating abundant genetic diversity existing among the cultivated radish. The 56 cultivars were clustered into 5 groups and 9 subgroups by cluster analysis, and 4 groups and 7 subgroups by principal coordinate analysis. The two methods above exhibited similar phylogenesis among the tested cultivars. The results from molecular classification were