原创力文档a rapid, high capacity assay for basic fibroblast growth factor binding：碱性成纤维细胞生长因子结合的快速、高容量的测定.pdfVIP
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a rapid, high capacity assay for basic fibroblast growth factor binding：碱性成纤维细胞生长因子结合的快速、高容量的测定
Life Sciences, Vol. 56, NO. 15, pp. 1257-1264, 1995
Pergamon Copyright Q 1995 Elsevier Science Ltd
Printed in the USA. All rights resewed
OQ24-3205/95 $9.50 + .oO
95)00071-2
A RAPID, HIGH CAPACITY ASSAY FOR BASIC FIBROBLAST
GROWTH FACTOR BINDING
W. F. Herblin+ and F. A. Barbera*
Cardiovascular Diseases Research
The DuPont Merck Pharmaceutical Company
Wilmington, DE 19880-0400
(Received in final form January 20, 199.5)
Summary
A rapid, high capacity assay for the binding of basic fibroblast growth factor
has been developed. Rat lung tissue was selected as the optimum source
of membranes and 2M sodium chloride used to remove endogenous
growth factor. The assay has been adapted to the Millipore Multi-Screen
system so that it can be run in 96-well format with a volume of 300 ul. The
assay has been validated through the demonstration of inhibition by
standard inhibitors such
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