大豆异黄酮抗氧作用的研究.pdfVIP

大豆异黄酮抗氧化作用的研究 摘 要 目的：研究大豆异黄酮对去卵巢大鼠和绝经后女性的抗氧化活性， 并探讨其作用机制。 方法：①3 月龄SD 雌性大鼠去卵巢术后按体重随机分为模型组和干 预组，每组8 只，分别用去离子水和大豆异黄酮提取物灌胃。于第13 周 末处死大鼠,透射电镜观察线粒体形态；氧电极法测定线粒体呼吸功能； 测定肝脏、脑氧化反应产物含量，肝、心和肾抗氧化酶活性。②绝经后 女性53 人，随机分为干预组和对照组，干预组每天口服大豆异黄酮60mg， 持续 6 个月。干预前后分别进行生活习惯、膳食习惯问卷调查，测定血 清氧化反应产物含量和抗氧化酶活性。 结果：①与模型组比较，干预组肝脏细胞线粒体的损伤变化有明显 改善；肝脏、脑细胞线粒体的呼吸功能增强；干预组脑细胞线粒体、肝 组织匀浆和细胞浆中MDA 含量较模型组显著下降，肝脏、心脏和肾组织 匀浆的GSH-Px 的活性较模型组显著增加。②社区研究中，干预组SOD 活 力无论与实验前比较，还是与对照组比较，都有明显增强（均为P0.05）； MDA 含量无论与实验前比较，还是与对照组比较，都有明显下降（分别 为P0.01 和P0.05）；GSH-Px 的变化不显著。 结论：大豆异黄酮能够提高去卵巢大鼠和绝经后女性的抗氧化能力。 关键词：大豆异黄酮，抗氧化，线粒体 1 STUDY ON ANTIOXIDANT EFFECT OF SOYBEAN ISOFLAVONES ABSTRACT Objective: To study the antioxidant effects of soy isoflavones on ovariectomized rats and postmenopausal women, and to explore the mechanisms. Methods: ①Three months old ovariectomized female SD rats were randomly divided into 2 groups (model and intervention groups), 8 in each group. They were gavaged with deionized water or soy isoflavones respectively. The rats were sacrificed on the 13th week. The mitochondrial morphology was observed with electron microscope. The mitochondrial respiratory function was assayed with the oxygen electrode. The oxidation product content and the antioxidant enzyme activity were determined.② Fifty-three postmenopausal women were randomly divided into control and intervention groups. The intervention group women were given a daily dose 60mg of soy isoflavones for six months. Before and after the intervention, the living habits, dietary surveys, serum oxidation