创复康喷剂对大创伤模型创面感染、体外抑菌及ctgf含量影响的研究.pdfVIP

摘 要 目的：通过观察创复康喷剂对大鼠感染模型创面中抗感染、体外抑菌及结缔组 织因子（CTGF）含量的影响，旨在从微生物学及分子生物学角度探讨创复康喷 剂对感染创面修复作用的机制。 方法：（1）30 只健康SD 大鼠，雌雄各半，随机分为A 生理盐水空白组、B 创复 康喷剂实验组、C 康复新液药物对照组，每组 10 只，称重标记。按照付小斌等 报道的背部全层皮肤切除感染模型制备方法并加以改进，制作大鼠感染模型。 （2）造模成功后开始分组外喷创复康喷剂、康复新液、生理盐水，5ml/次，每 日2 次，连续用9 日。在治疗第1d、5d、9d，分别于创面中心及边缘各切取一 痂下组织或肉芽组织，制成组织匀浆，进行菌落计数。用药第 9d，切取未愈 合创面边缘 0.3cm 的新生肉芽组织和皮肤，每只大鼠取约0.5g，超声波粉碎 后，制成组织匀浆，将制备好的匀浆离心留上清液，采用双抗体两步夹心酶 联免疫吸附法（ELIsA）检测CTGF 浓度。 结果：（1）创复康喷剂组和康复新液组均能能降低感染创面中细菌含量，无 显著性差异，在治疗后第5d、9d，创复康喷剂组和生理盐水组的细菌含量有 显著性差异。表明运用创复康喷剂可以降低感染创面中细菌含量，有抗感染 的作用。 （2）用药9d 后，创复康喷剂组和康复新剂组的CTGF 含量无显著性差异， 创复康喷剂组与生理盐水组的CTGF 含量有显著性差异。 结论：创复康喷剂能够降低感染创面中细菌含量，能促进感染创面的愈合,提 高CTGF 的含量。 关键词：创复康喷剂；全层皮肤切除模型；体外抑菌；CTGF；细菌含量 3 The content of the bacterial and (CTGF) affected by wound healing spray on the wound on wound infection in rats Abstract Objective：Record of rehabilitation by observing the spray on the wound infection model in rat anti-infective, antibacterial and connective tissue factor (CTGF) content, designed from the perspective of microbiology and molecular biology of invasive wound infection rehabilitation spray repair mechanism. Methods: (1) 30 healthy SD rats were randomly divided into saline control group A, B Chong Rehabilitation spray the experimental group, C rehabilitation new fluid control group, n = 10, weighing tag. Such reports in accordance with Fu Xiaobin back full thickness skin excision infection model and improved preparation, rat infection model.(2) After the modeling group and Spraying started spray invasive rehabilitation, rehabilitation of new fluid, normal saline, 5ml / times, 2 times a day for successive 9. In the treatment section 1d, 5d, 9d, respectively, the edge of the wound center and take a cut or granulation tissue under eschar, made of tissue, for colony coun