电针夹脊穴对缺再灌注损伤大鼠心肌细胞凋亡及bcl-2、bax蛋白表达的影响.pdfVIP

蛋白表达量都较少，模型组心肌 Bcl-2、Bax 蛋白表达量较假手术组均明显增加 (P0.01)，其中以 Bax蛋白表达更明显。电针夹脊组和内关组中Bax蛋白表达较模型 组显著下降(P0.01)，但Bcl-2蛋白的表达量较模型组显著升高(P0.01)。电针夹脊 组和内关组中的 Bcl-2、Bax 值无显著性差异（P＞0.05），但二者与假手术组有显著 性差异(P0.01)。 结论：电针夹脊穴对缺血再灌注损伤心肌有明显的保护作用。其机制可能与提高 缺血再灌注损伤大鼠血清中NO含量，降低ET、IL-1β含量，提高SOD含量，降低MDA 含量以及通过调节Bcl-2、Bax等凋亡调控基因的表达抑制细胞凋亡密切相关。 关键词 夹脊穴；心肌缺血再灌注；内皮功能；抗氧化作用；细胞凋亡；Bcl-2； Bax The Effects of Electro- acupuncture of “jiaji-acupoints”on Cardiomyocytes Apotosis and the expression of apotosis related protein BCL-2 and BAX on ischemia/ reperfusion in Rats Speciality:Acupuncture and Moxibustion Author: Kong Suping Tutor: Tan Qiwen ABSTRACT Objective：To investigate the protective effects of electro-acupuncture of “jiajixue” on myocardial ischemia and reperfusion injury in rats ，endothelial function、antioxidation、 the extent of apoptosis in cardiomyocytes ,and the expression of Bcl-2/Bax protein ，to explore the possible protective mechanism of acupuncture of “jiajixue” on myocardial ischemia and reperfusion injury. Methods ：50 wistar rats were randomly divided into 5 groups: Jiaji group, Neiguan group, model group, sham-operation group, Yanglingquan group ,with 10 rats in each group. Myocardial ischemia and reperfusion injury model was duplicated by occlusion of the descending anterior branch of the left coronary artery. The content of NO、ET 、IL-1 β、 SOD、MDA in serum were measured by ELISA. Apoptotic myocytes were detected with the TUNEL method. The expression of Bcl-2 and Bax protein in myocardial cells was detected by immmunohistochemistry. Results ：1. Compared with the sham-operation, NO content significantly decreased （p ﹤0.01 ）, IL-1β content significantly increased in the other group （p ﹤0.01 ）;ET content significantly increased in model and Yanglingquan groups （p