贵州小型猪胆汁积性肝纤维化模型的建立.pdfVIP

9 白细胞（WBC ）在术后7d 即升高（30.0±5.7 ）×10 /L ，与术前（11.7±1.8 ） 9 ×10 /L 相比差异明显（P0.01 ），红细胞（RBC ）与血红蛋白（HGB ）在 术后28d 里缓慢下降，与术前相比有差异（P0.05 ）；凝血酶原时间（PT ）、 凝血酶时间（TT ）及纤维蛋白原（DFbg ）在术后28d 里缓慢下降，与术 前相比有明显差异（P0.01 ）；HE 染色见，手术后肝小叶结构逐渐破坏， 小叶间纤维增生、排列紊乱。Masson 染色结果，术后14d,7 头猪中6 头肝 纤维化为S1 级，1 头S2 级，28d 成活的7 头猪肝纤维化均达S3 级；电 镜提示在术后 7d，肝细胞内出现大量脂滴，粗面内质网消失，核周隙增 宽，核膜皱缩，线粒体水肿；病理检测及免疫组化显示：在胆总管结扎术 后 14d，肝细胞凋亡增多，Fas 表达增加，肝纤维化程度加重。结论：通 过结扎贵州小型猪胆总管可成功建立胆汁淤积性肝纤维化模型；其肝纤维 化的发生发展可能与Fas 表达的增加，促进肝细胞凋亡有关。 关键词：肝纤维化；胆汁淤积；猪；模型；细胞凋亡 2 2 The establishment of porcine cholestatic liver fibrosis model Abstract: Objective: To establish cholestatic liver fibrosis model in Guizhou miniature pigs by bile duct ligation. To observe the changes of liver function and histopathology in the model animals. Detection of Fas protein expression and apoptosis, objective evaluation model and to investigate the possible mechanisms of fibrosis development. Methods: Eight female Guizhou miniarure pigs, weighting 12 to 15 kg, were selected as experiment animals. 3% pentobarbital (1ml/kg) was injected into the body of pig by intraperitoneal anesthesia. The common bile duct was revealed by laparotomy. The common bile duct was double ligated and transected between the two lines. The animal model was established by this way. Blood samples were taken respectively at preoperative 30min, 7th day, 14th day and 28th day after the operation for liver function test, blood test and blood coagulation function test. Liver tissue biopsy was done respectively for histopathological light microscope and electron microscope examination before bile duct ligation and postoperative 7th day, 14th day and 28th day. Fas protein and cell apoptosis in hepatocytes were tested by immunohistochemistry.